Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1.: Anti-allergen IgG can enhance the anaphylactic reaction
Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1.: Anti-allergen IgG can enhance the anaphylactic reaction
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DOI：
10.1016/s0014-5793(99)01703-2
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发表时间：
2000-01-07
期刊：
影响因子：
3.5
通讯作者：
Lebecque, S
中科院分区：
文献类型：
--
作者：
Denépoux, S;Eibensteiner, PB;Lebecque, S
我们报道了5种人单克隆抗体BAB1-5 (BAB1: IgG(1)；BAB4:免疫球蛋白g (2);BAE2, 3,5: IgG(4))，对桦树花粉主要过敏原betv1具有特异性。BAB1-5是在免疫治疗后获得的，含有高度的体细胞突变，表明抗原驱动的亲和成熟过程。虽然BAB1抑制了患者IgE与Bet v1的结合，但BAB2增加了IgE对Bet v1的识别，并且，即使大肠杆菌表达Fab，也增强了Bet v1诱导的即时型皮肤反应。IgG抗体可以增强过敏原诱导的过敏反应，这可能解释了特异性免疫治疗的不可预测性。(C) 2000年欧洲生化学会联合会。
We report the molecular characterization of five human monoclonal antibodies, BAB1-5 (BAB1 : IgG(1) ; BAB4: IgG(2); BAE2, 3, 5: IgG(4)), with specificity for the major birch pollen allergen, Bet v 1. BAB1-5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy. (C) 2000 Federation of European Biochemical Societies.