Microhabitat heterogeneity across leaves and flower organs promotes bacterial diversity
Microhabitat heterogeneity across leaves and flower organs promotes bacterial diversity
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DOI：
10.1093/femsec/fiv097
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发表时间：
2015-09-01
影响因子：
4.2
通讯作者：
Keller, Alexander
中科院分区：
文献类型：
--
作者：
Junker, Robert R.;Keller, Alexander
真核生物相关微生物组以多种方式与宿主相互作用，从而影响宿主的表型、身体状况和行为。在植物中，细菌具有多种功能，在自然和农业系统中具有可变的净效应。然而，与不同的地上植物器官，特别是花相关的细菌群落的组成和多样性的信息是缺乏的。此外，微生境和环境条件对群落建立的相对影响需要进一步关注。在这里，使用文化独立的方法，我们确定的叶子和三个花的微生境（花蜜，耐力和风格）的Metrosideros polymorpha（桃金娘科），夏威夷特有的树，主机独特的指示社区组成的相对丰富的细菌类群。这些指示菌群伴随着大量普遍存在或丰度较低的稀有细菌。在我们的研究系统中，微生境过滤对植物相关群落组成和细菌丰富度和多样性的强烈影响远远超过了环境效应，如降水，海拔，基质年龄和地理距离的影响。因此，地上植物器官的细菌丰富度被严重低估时，只考虑一个微生境，如叶。我们的研究是全面表征植物细菌群落的分布、组成和潜在因素的第一步，对未来植物健康、授粉和繁殖的基础和应用研究具有重要意义。
Eukaryote-associated microbiomes interact with their hosts in multiple manners, thereby affecting the hosts' phenotype, physical condition and behaviour. In plants, bacteria have numerous functions, with variable net effects, both in natural and agricultural systems. However, information about the composition and diversity of the bacterial communities associated with different aboveground plant organs, particularly flowers, is lacking. In addition, the relative effects of microhabitat and environmental conditions on community establishment require further attention. Here, using culture-independent methods, we determine that leaves and three floral microhabitats (nectar, stamina and styles) of Metrosideros polymorpha (Myrtaceae), a tree endemic to Hawai'i, host unique indicator communities composed of relatively abundant bacterial taxa. These indicator communities are accompanied by a large number of ubiquitous or rare bacteria with lower abundances. In our study system, the strong effect of microhabitat filtering on plant-associated community composition and bacterial richness and diversity strongly exceeds the influence of environmental effects such as precipitation, altitude, substrate age and geographic distance. Thus, the bacterial richness of aboveground plant organs is strongly underestimated when only one microhabitat, e.g. leaves, is considered. Our study represents a first step towards a comprehensive characterization of the distribution, composition and underlying factors, of plant bacterial communities, with implications for future basic and applied research on plant health, pollination and reproduction.