Development of Indica Basmati rice harboring two insecticidal genes for sustainable resistance against lepidopteran insects
Development of Indica Basmati rice harboring two insecticidal genes for sustainable resistance against lepidopteran insects
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DOI：
10.1016/j.sajb.2005.07.005
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发表时间：
2006-05-01
影响因子：
3.1
通讯作者：
Riazuddin, S.
中科院分区：
文献类型：
--
作者：
Riaz, N.;Husnain, T.;Riazuddin, S.
将携带cry1Ac和cry2A基因的共整合载体pSM 6转化水稻品种Basmati 370。将单独载体pROB 5上的潮霉素抗性基因用作选择标记。用生物射弹枪转化1至2周龄的盾片来源的愈伤组织。通过PCR、Dot blot、Southern blot、Western blot和ELISA等分子生物学分析方法，以及水稻纵卷叶螟（RLF）和二化螟（YSB）的取食试验，对不同转化系的转基因状态进行了评价。在一组实验中观察到1.98%的平均转化效率。来自五个不同的转基因株系的植株对稻纵卷叶螟表现出100%的死亡率，并以孟德尔方式分离。昆虫生物测定结果表明，cry1Ac和cry2A基因在抗性发展过程中没有协同或拮抗作用。(c)2005款萨博。Elsevier B.V.出版，保留所有权利。
Rice variety Basmati 370 was transformed with a co-integrate vector pSM6 carrying cry1Ac and cry2A genes. The hygromycin resistance gene on a separate vector, pROB5 was used as a selectable marker. One to two week old scutellum derived calli were transformed with a biolistic gun. Transgenic status of different transformed lines was evaluated by molecular analysis (PCR, Dot blot, Southern blot, Western blot and ELISA) and insect feeding assays with rice leaf folder (RLF) and yellow stem borer (YSB). An average transformation efficiency of 1.98% was observed in a set of experiments. Plants from five different transgenic lines exhibited 100% mortality against rice leaf folder and segregated in Mendelian fashion. Insect bioassays revealed that there is no synergistic or antagonistic effect of cry1Ac and cry2A genes on the development of resistance against RFL and YSB. (c) 2005 SAAB. Published by Elsevier B.V. All rights reserved.