Angiofil®-mediated visualization of the vascular system by microcomputed tomography:: A feasibility study
Angiofil®-mediated visualization of the vascular system by microcomputed tomography:: A feasibility study
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DOI：
10.1002/jemt.20585
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发表时间：
2008-07-01
影响因子：
2.5
通讯作者：
Djonov, Valentin
中科院分区：
文献类型：
--
作者：
Grabherr, Silke;Hess, Andreas;Djonov, Valentin
器官或小动物血管系统的可视化对于基本生理状况的评估是重要的，特别是在涉及转基因小鼠的研究中。为了对维管树进行详细的形态学分析，有必要对整个系统进行演示。在这项研究中，我们提出了一种新的亲脂性造影剂Angiofil (R)，用于通过微计算机断层扫描进行死后微血管造影。在10只野生型小鼠身上进行了新型造影剂的试验。血管系统的成像显示血管小到毛细血管的直径，从扫描中获得的数字三维数据允许在不破坏样品的情况下进行虚拟切割、放大和缩放。通过使用计算机软件，血管长度和口径等参数可以量化，并通过颜色编码重新映射到血管系统表面。液体血管膜(R)易于处理，具有高度的放射性不透明。由于其亲脂性，它被保留在血管内，因此它有助于虚拟血管分割，并产生持久的信号，这在重复调查中是有利的，或者如果样品需要从制备地点运送到实际分析地点。这些特点使Angiofil (R)成为一种很有前途的新型造影剂；当与微计算机断层扫描相结合时，它有可能成为快速血管表型的有力方法。
Visualization of the vascular systems of organs or of small animals is important for an assessment of basic physiological conditions, especially in studies that involve genetically manipulated mice. For a detailed morphological analysis of the vascular tree, it is necessary to demonstrate the system in its entirety. In this study, we present a new lipophilic contrast agent, Angiofil (R), for performing postmortem microangiography by using microcomputed tomography. The new contrast agent was tested in 10 wild-type mice. Imaging of the vascular system revealed vessels down to the caliber of capillaries, and the digital three-dimensional data obtained from the scans allowed for virtual cutting, amplification, and scaling without destroying the sample. By use of computer software, parameters such as vessel length and caliber could be quantified and remapped by color coding onto the surface of the vascular system. The liquid Angiofil (R) is easy to handle and highly radio-opaque. Because of its lipophilic abilities, it is retained intravascularly, hence it facilitates virtual vessel segmentation, and yields an enduring signal which is advantageous during repetitive investigations, or if samples need to be transported from the site of preparation to the place of actual analysis, respectively. These characteristics make Angiofil (R) a promising novel contrast agent; when combined with microcomputed tomography, it has the potential to turn into a powerful method for rapid vascular phenotyping.