Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex
Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex
复制标题
DOI：
10.1128/jcm.43.9.4665-4673.2005
复制
发表时间：
2005-09-01
影响因子：
9.4
通讯作者：
Dowson, CG
中科院分区：
文献类型：
--
作者：
Baldwin, A;Mahenthiralingam, E;Dowson, CG
开发了单多位点序列分型 (MLST) 方案，用于精确表征洋葱伯克霍尔德菌复合体 (BCC) 的机会性病原体，该群体由至少九种密切相关的物种组成。在对五个伯克霍尔德氏菌属物种进行比较后，选择了七个保守的管家基因，并使用巢式PCR扩增方法对每个基因的菌株集合进行了核苷酸序列分析。 MLST 区分了所有 9 个当前 BCC 物种，并在 119 个菌株的集合中鉴定了 114 个序列类型。从环境或临床来源回收的菌株之间没有发现差异。 MLST 提供的菌株鉴定分辨率得到了提高，能够识别先前表征的流行菌株谱系，并证明复合体中存在四个新的潜在物种组。还有证据表明重组在单个 BCC 物种的近期进化中发挥着重要作用。这种高度可转移、经过验证的 MLST 方案提供了一种新的方法来协助物种鉴定以及通过单一方法对 BCC 进行明确的菌株区分。这也是第一个从一开始就设计的包含多个物种的 MLST 计划，应有助于 BCC 的全球流行病学调查。
A single multilocus sequence typing (MLST) scheme was developed for precise characterization of the opportunistic pathogens of Burkholderia cepacia complex (BCC), a group composed of at least nine closely related species. Seven conserved housekeeping genes were selected after a comparison of five Burkholderia species, and a collection of strains was subjected to nucleotide sequence analysis using a nested PCR amplification approach for each gene. MLST differentiated all nine current BCC species and identified 114 sequence types within a collection of 119 strains. No differentiation was found between strains recovered from environmental or clinical sources. The improved resolution in strain identification offered by MLST was able to identify previously characterized epidemic strain lineages and also demonstrated the presence of four novel potential species groups within the complex. There was also evidence for recombination having an important role in the recent evolution of individual BCC species. This highly transferable, validated, MLST scheme provides a new means to assist in species identification as well as unambiguous strain discrimination of the BCC by a single approach. It is also the first MLST scheme designed at the outset to incorporate multiple species and should facilitate global epidemiological investigations of the BCC.