1H magnetic resonance imaging of freezing and thawing in freeze-tolerant frogs.
1H magnetic resonance imaging of freezing and thawing in freeze-tolerant frogs.
复制标题
耐冻青蛙冷冻和解冻的 1H 磁共振成像。
DOI：
10.1152/ajpregu.1994.266.6.r1771
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Storey,KB
中科院分区：
文献类型：
--
作者：
Rubinsky,B;Wong,ST;Hong,JS;Gilbert,J;Roos,M;Storey,KB
利用质子磁共振成像技术（MRI）对林蛙（Rana sylvatica）的冷冻和解冻过程进行了无创、实时的分析，分析了冰在这种耐寒脊椎动物体内的传播模式。核磁共振成像显示，冰从外部向内定向移动，需要几个小时才能完成。产生和输出冷冻保护剂的肝脏和循环冷冻保护剂的心脏等核心器官的冷冻被器官外冰包围后才开始。自然解冻是一个完全不同的过程；整个身体开始均匀地融化，但核心器官比外围器官融化得更快，这种适应可能是早期恢复心跳和呼吸的关键。所展示的图像显示了MRI的灵敏度和能力，以及它在哺乳动物器官外植体冷冻保存技术发展中成为关键监测技术的潜力。
Proton magnetic resonance imaging (MRI) of the processes of freezing and thawing in the wood frog Rana sylvatica provided noninvasive and real-time analysis of the mode of ice propagation through the body of a freeze-tolerant vertebrate. MRI revealed a directional movement of ice from the exterior inward that required several hours to reach completion. Freezing in core organs such as liver, which produces and exports cryoprotectant, and heart, which circulates it, was delayed and occurred well after the organs were surrounded by extraorgan ice. Natural thawing was a very different process; thawing began uniformly throughout the body, but core organs melted more rapidly than peripheral ones, an adaptation that may be key to the early restoration of heartbeat and breathing. The images presented demonstrate the sensitivity and power of MRI and its potential to become a critical monitoring technology in the development of cryopreservation techniques for mammalian organ explants.