Multiplexed Detection of Antibodies Using Programmable Bead Arrays
Multiplexed Detection of Antibodies Using Programmable Bead Arrays
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DOI：
10.1007/978-1-61779-043-0_15
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Anderson, Karen S.
中科院分区：
文献类型：
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作者：
Anderson, Karen S.
血清中抗体的检测在传染病、自身免疫和癌症的检测和监测方面具有广泛的应用。抗原检测的蛋白质组学方法（例如蛋白质微阵列）是出色的临床发现工具，但由于成本和制造的专业化，这些方法仅限于筛选少量血清。生物标志物验证研究的下游测定需要快速、可重复的多重测定，以使用数百或数千份血清同时筛选较少（< 100）的抗原。传统的临床 ELISA 检测使用重组蛋白，但这些检测受到纯化不含交叉反应污染物的蛋白的能力的限制，并且一次仅限于一种抗原。在此，WC 描述了将体外蛋白质生产与微珠阵列上的抗标签捕获相结合的应用，用于快速多重检测血清中的抗体。这些测定可以很容易地适用于检测任何蛋白质特异性感染性抗体、自身免疫性抗体或癌症特异性抗体。
The detection of antibodies in sera has broad applications for detection and monitoring of infectious diseases, autoimmunity, and cancer. Proteomic methods of antigen detection, such as protein microarrays, are excellent clinical discovery tools, but due to both cost and specialization of manufacture, these are limited to screening small numbers of sera. Downstream assays for biomarker validation studies require rapid, reproducible, multiplexed assays for the simultaneous screening of fewer (< 100) antigens with hundreds or thousands of sera. Traditional clinical ELISA assays use recombinant proteins, but these are limited by the ability to purify proteins free of cross-reacting contaminants and are limited to one antigen at a time. Here, WC describe the application of coupled in vitro protein production with anti-tag capture onto bead arrays, for the rapid multiplexed detection of antibodies in sera. These assays can be readily adapted for detection of any protein-specific infectious, autoimmune, or cancer-specific antibodies.