PADE-LAPLACE METHOD FOR ANALYSIS OF FLUORESCENCE INTENSITY DECAY
PADE-LAPLACE METHOD FOR ANALYSIS OF FLUORESCENCE INTENSITY DECAY
复制标题
DOI：
10.1016/s0006-3495(89)82653-0
复制
发表时间：
1989-07-01
影响因子：
3.4
通讯作者：
PRENDERGAST, FG
中科院分区：
文献类型：
--
作者：
BAJZER, Z;MYERS, AC;PRENDERGAST, FG
这种分析多指数函数的新方法是基于拉普拉斯变换和Pade逼近(Yeramian，E.和P.Claverie)的组合使用的。1987年。自然(长)。326：169-174)。它在原理上类似于著名的Isenberg矩方法(Isenberg，I.1983)。生物群落。J.43：141-148)传统上应用于荧光衰变的分析。Pade-Laplace方法的优点在于它能够检测多指数函数中的分量数量以及它们的参数。本文对原方法进行了改进，使之适用于荧光衰变的多频相位/调制测量。该方法首先在模拟数据上进行了检验。它提供了最多四个不同的寿命组分(及其部分贡献)的回收。在模拟数据对应于连续寿命分布(非指数衰减)的情况下，Pade-Laplace方法的分析结果表明不存在离散指数分量。该方法也被应用于从已知的荧光团及其混合物和磷脂酶A2中的色氨酸荧光收集的真实相位/调制数据。寿命和分数的回收率与标准的非线性最小二乘拟合法一致。
This novel approach to the analysis of mulitexponential functions is based on the combined use of the Laplace transform and Pade approximants (Yeramian, E., and P. Claverie. 1987. Nature (Lond.). 326:169-174). It is similar in principle to the well-known Isenberg method of moments (Isenberg, I. 1983. Biophys. J. 43:141-148) traditionally applied to the analysis of fluorescence decay. The advantage of the Pade-Laplace method lies in its ability to detect the number of components in a multiexponential function as well as their parameters. In this paper we modified the original method so that it can be applied to the analysis of multifrequency phase/modulation measurements of fluorescence decay. The method was tested first on simulated data. It afforded recovery up to four distinct lifetime components (and their fractional contributions). In the case of simulated data corresponding to continuous lifetime distributions (nonexponential decay), the results of the analysis by the Pade-Laplace method indicated the absence of discrete exponential components. The method was also applied to real phase/modulation data gathered on known fluorophores and their mixtures and on tryptophan fluorescence in phospholipase A2. The lifetime and fraction recoveries were consistent with those obtained from standard methods involving nonlinear least-square fitting.