High-performance liquid chromatography analysis of curcumin in rat plasma: application to pharmacokinetics of polymeric micellar formulation of curcumin
High-performance liquid chromatography analysis of curcumin in rat plasma: application to pharmacokinetics of polymeric micellar formulation of curcumin
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DOI：
10.1002/bmc.795
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发表时间：
2007-05-01
影响因子：
1.8
通讯作者：
Samuel, John
中科院分区：
文献类型：
--
作者：
Ma, Zengshuan;Shayeganpour, Anooshirvan;Samuel, John
建立了一种简便、快速、可靠的测定大鼠血浆中姜黄素浓度的高效液相色谱法。加入内标物（IS）4-羟基二苯甲酮后，用乙腈沉淀血浆。采用沃茨mu Bondapak（TM）C-18柱（3.9 × 300 mm，5 4 μ m），乙腈（55%）和柠檬酸缓冲液（pH 3.0）（45%）为移动的流动相，流速为1.0 mL/min。检测波长分别为300 nm和428 nm。对50、200和1000 ng/mL姜黄素的提取率分别为97.08、95.69和94.90%。线性范围为0.02-1 μ g/mL，相关系数r（2）> 0.999。在50、200和1000 ng/mL姜黄素浓度下，日内和日间变异系数小于13%，平均日内和日间误差小于+/- 6%。该方法已成功应用于姜黄素及其聚合物胶束制剂在大鼠体内的药代动力学研究。发现聚合物胶束使姜黄素的半衰期增加至溶解的姜黄素的162倍，并且使分布容积（Vd（ss））增加至70倍。版权所有（c）2007约翰威利父子有限公司。
A simple, rapid and reliable high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of curcumin in rat plasma. Plasma was precipitated with acetonitrile after addition of the internal standard (IS), 4-hydroxybenzophenone. Separation was achieved on a Waters mu Bondapak (TM) C-18 column (3.9 x 300mm, 5 4 mu m) using acetonitrile (55%) and citric buffer, pH 3.0 (45%) as the mobile phase (flow rate = 1.0 mL/min). The UV detection wavelength was 300 and 428 nm for IS and curcumin, respectively. The extraction efficiencies were 97.08, 95.69 and 94.90% for 50, 200 and 1000 ng/mL of curcumin in rat plasma, respectively. The calibration curve was linear over the range 0.02-1 mu g/mL with a correlation coefficient of r(2) > 0.999. The intra- and inter-day coefficients of variation were less than 13%, and mean intra- and inter-day errors were less than +/- 6% at 50, 200 and 1000 ng/mL of curcumin. This assay was successfully applied to the pharmacokinetic studies of both solubilized curcumin and its polymeric micellar formulation in rats. It was found that polymeric micelles increased the half-life of curcumin 162-fold that of solubilized curcumin and increased the volume of distribution (Vd(ss)) by 70-fold. Copyright (c) 2007 John Wiley & Sons, Ltd.