Cholesterol-loaded cyclodextrin enhances osmotic tolerance and inhibits the acrosome reaction in rabbit spermatozoa
Cholesterol-loaded cyclodextrin enhances osmotic tolerance and inhibits the acrosome reaction in rabbit spermatozoa
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DOI：
10.1016/j.anireprosci.2010.02.014
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发表时间：
2010-07-01
影响因子：
2.2
通讯作者：
Erdem, Hueseyin
中科院分区：
文献类型：
--
作者：
Aksoy, Melih;Akman, Orhan;Erdem, Hueseyin
在两个连续的实验中，研究了胆固醇-环糊精（CLC）处理对兔精子的渗透耐受性和顶体反应能力的影响，所述精子在质膜中具有异常高的胆固醇/磷脂比。在第一个实验中，CLC预处理和未处理的精子细胞暴露于五种果糖溶液中的一种15分钟，调节至20，80，290，500或1500 mOsm/L。各向异性激发后，通过改良的低渗肿胀试验（HOST）和超活体伊红染色试验（HE试验）评估补充CLC组（剂量水平为3 mg/120 x 10（6）个精子）和对照组中精子膜的完整性。在第二部分研究中，我们观察了胆固醇对钙离子载体A23187（CI）或溶血磷脂酰胆碱（LPC）刺激的精子顶体反应的影响，CLC预处理可提高精子的活率和对活的HOST反应的精子率（P0.05）。CLC预处理和渗透压水平在维持精子膜的功能和物理完整性方面存在显著的相互作用。CI和LPC均能诱导家兔精子顶体反应（P
The effects of cholesterol-loaded cyclodextrin (CLC) treatment on the osmotic tolerance and ability to undergo the acrosome reaction of rabbit spermatozoa, with an unusually high cholesterol/phospholipid ratio in plasma membranes, were examined in two successive experiments. In the first experiment, CLC-pretreated and untreated sperm cells were exposed for 15 min to one of five fructose solutions, adjusted to 20, 80, 290, 500 or 1500 mOsm/L. After the anisoosmotic challenge, the integrity of sperm membranes in the CLC-supplemented (at a dose level of 3 mg/120 x 10(6) spermatozoa) and control groups was estimated by a modified hypoosmotic swelling test (HOST) associated with a supravital eosin staining test (HE-test). In the second part of the study, the influence of cholesterol supplementation on the acrosome reaction of sperm cells stimulated by either calcium ionophore A23187 (CI) or lysophosphatidylcholine (LPC) was evaluated.CLC pretreatment increased viable and live-HOST-responsive sperm rates (P0.05). A significant interaction was determined between CLC pretreatment and the level of osmotic pressure in maintaining the functional and physical integrities of sperm membranes undergoing osmotic challenges. Both CI and LPC successfully induced the acrosome reaction in rabbit spermatozoa (P