Expression patterns, mutation detection and RNA interference of Rhopalosiphum padi voltage-gated sodium channel genes.
Expression patterns, mutation detection and RNA interference of Rhopalosiphum padi voltage-gated sodium channel genes.
复制标题
稻瘟病菌电压门控钠通道基因的表达模式、突变检测和RNA干扰
DOI：
10.1038/srep30166
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发表时间：
2016-07-21
影响因子：
4.6
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Zuo Y;Peng X;Wang K;Lin F;Li Y;Chen M
电压门控钠通道（VGSC）是钠通道阻断剂的作用靶点。传统上，动物被认为只有一个VGSC基因，包括一个α亚基和四个同源结构域（DI-DIV）。研究表明，禾谷缢管蚜（Rhopalosiphum padi）是一种重要的农作物害虫，具有一个独特的异二聚体VGSC（H1和H2亚基），由两个基因（Rpvgsc 1和Rpvgsc 2）编码，这在昆虫和其他动物中是不常见的。Rpvgsc 1的开放阅读框（ORF）由1150个氨基酸组成，Rpvgsc 2的ORF由957个氨基酸组成。Rpvgsc 1与果蝇VGSC的DI-DII具有64.1%的氨基酸同一性，Rpvgsc 2与果蝇DIII-DIV具有64.0%的氨基酸同一性。黑腹果蝇在拟除虫菊酯类杀虫剂抗性品系中发现了一个M918 L突变，该突变位于拟除虫菊酯类杀虫剂抗性品系的IIS 4-S6区域。稻田取样。两个R。水稻VGSC基因在所有发育阶段都有表达，在用高效氯氰菊酯处理后表现出相似的表达模式。Rpvgsc 1和Rpvgsc 2基因的敲除可显著降低R.暴露于高效氯氰菊酯后的PADI。这些结果表明，两个R。水稻VGSC基因是两个功能基因。
The voltage-gated sodium channel (VGSC) is the target of sodium-channel-blocking insecticides. Traditionally, animals were thought to have only one VGSC gene comprising a α-subunit with four homologous domains (DI–DIV). The present study showed that Rhopalosiphum padi, an economically important crop pest, owned a unique heterodimeric VGSC (H1 and H2 subunits) encoded by two genes (Rpvgsc1 and Rpvgsc2), which is unusual in insects and other animals. The open reading frame (ORF) of Rpvgsc1 consisted 1150 amino acids, and the ORF of Rpvgsc2 had 957 amino acids. Rpvgsc1 showed 64.1% amino acid identity to DI–DII of Drosophila melanogaster VGSC and Rpvgsc2 showed 64.0% amino acid identity to DIII–DIV of D. melanogaster VGSC. A M918L mutation previously reported in pyrethroids-resistant strains of other insects was found in the IIS4-S6 region of R. padi field sample. The two R. padi VGSC genes were expressed at all developmental stages and showed similar expression patterns after treatment with beta-cypermethrin. Knockdown of Rpvgsc1 or Rpvgsc2 caused significant reduction in mortality rate of R. padi after exposure to beta-cypermethrin. These findings suggest that the two R. padi VGSC genes are both functional genes.