Fecal microflora of Greek healthy neonates
Fecal microflora of Greek healthy neonates
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DOI：
10.1016/j.anaerobe.2007.11.002
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发表时间：
2008-04-01
期刊：
影响因子：
2.3
通讯作者：
Kyriacou, Adamantini
中科院分区：
文献类型：
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作者：
Mitsou, Evdokia K.;Kirtzalidou, Ekaterini;Kyriacou, Adamantini
本研究旨在探讨我国地理区域婴儿出生后头三个月肠道菌群的发育、乳酸菌和双歧杆菌的定植模式，以及分娩方式的影响。本研究前瞻性地收集了82例健康足月婴儿在分娩后4、30和90天的粪便标本，并在非选择性和选择性培养基上进行传代培养。采用微生物学和分子学方法对分离菌株进行鉴定。对于分娩效果，研究了两组阴道分娩或剖宫产的纯母乳喂养婴儿。尽管早期需氧菌和厌氧菌总数很高，但乳酸菌和双歧杆菌的定植总体上受到限制，直到3个月大。此外，与顺产婴儿相比，剖腹产婴儿在第4天感染乳酸菌（4%对59%，p=0.000）、第4天感染双歧杆菌（0%对23%，p=0.015）和30（0%对35%，p=0.042）的情况较少。没有检测到细菌种群差异来比较定植的婴儿。鉴定结果显示，在出生后第一个月，鼠李糖乳杆菌、约氏乳杆菌和副干酪乳杆菌在新生儿肠道菌群中占主导地位，而在第4天，经阴道分娩的定植新生儿中，乳酸菌种类具有多样性。此外，长双歧杆菌和短双歧杆菌是阴道分娩和母乳喂养婴儿中最常见的双歧杆菌种类。总之，我们的研究揭示了希腊婴儿中乳酸菌的有限定植模式和剖腹产后乳酸菌和双歧杆菌菌群发育的延迟。需要进一步分析这些发现的潜在后果。(c) 2007 Elsevier Ltd.版权所有。
This study aimed to explore, in our geographical region, the development of intestinal microflora and the colonization patterns of lactic acid bacteria and bifidobacteria during the first three months of life and to investigate the effect of the mode of delivery. Fecal specimens from 82 healthy, full-term infants were collected prospectively 4, 30 and 90 days after delivery and subcultured on nonselective and selective media. Identification of isolates was performed by microbiological and molecular methods. For the delivery effect, two groups of vaginally or caesarean-delivered exclusively breast-fed infants were studied. Despite the early high total counts of aerobes and anaerobes, colonization of lactobacilli and bifidobacteria was overall limited until 3 months of age. Furthermore, caesarean-delivered infants were less often colonized with lactobacilli at day 4 (4% vs. 59%, p=0.000) and with bifidobacteria at day 4 (0% vs. 23%, p=0.015) and 30 (0% vs. 35%, p=0.042) compared to vaginally delivered ones. No bacterial populations differences were detected to compare colonized infants. Identification results indicated the predominance of Lactobacillus rhamnosus, Lactobacillus johnsonii and Lactobacillus paracasei species in neonatal gut microflora up to the first month of life and diversity of Lactobacillus species in vaginally delivered, colonized newborns, at fourth day. Furthermore, Bifidobacterium longum and Bifidobacterium breve were the most frequently detected Bifidobacterium species in vaginally delivered, breast-fed infants. In conclusion our study revealed a restricted colonization pattern of lactic acid bacteria in Greek infants and a delay in the development of Lactobacillus and Bifidobacterium spp. microbiota after caesarean section. Further analysis of potential consequences of these findings is required. (c) 2007 Elsevier Ltd. All rights reserved.