Metabolic proxy for cephalopods: Stable carbon isotope values recorded in different biogenic carbonates
Metabolic proxy for cephalopods: Stable carbon isotope values recorded in different biogenic carbonates
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DOI：
10.1111/2041-210x.13630
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发表时间：
2021-05-29
影响因子：
6.6
通讯作者：
Wang, Chia-Hui
中科院分区：
文献类型：
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作者：
Chung, Ming-Tsung;Chen, Ching-Yi;Wang, Chia-Hui
测量海洋动物在自然环境中的代谢率具有挑战性，阻碍了我们对其生理生态的理解。最近，一种新的代谢代理，即三角洲碳13值的生物碳酸盐（耳石），在硬骨鱼实验验证。头足类具有几种类型的生物碳酸盐，如耳石、乌贼骨和其他内、外壳，它们都是潜在的代谢记录器，但很少被评估。我们用乌贼Sepia pharaonis进行了一个温度控制实验，以评估代谢衍生碳（C-resp）的比例是否在温暖的环境中，随着新陈代谢的增加，耳石和乌贼骨中的铁含量也会增加。此外，我们还利用已发表的各种生物碳酸盐的δ C-13值进行了多物种分析，以评估头足类物种之间以及头足类和硬骨鱼类之间的替代物的一致性。平衡石和乌贼骨之间的C-呼吸值没有差异。此外，头足类生物碳酸盐的C-resp值与鱼类耳石的C-resp值范围相似，并与环境温度、体重、个体发育和功能行为等代谢相关因素相关，加强了其应用的可行性。它可以转化为耗氧率，用于与其他物种进行更广泛的比较，并用于与代谢生态学相关的当前理论进行进一步评估。然而，强烈建议进行实验验证，因为来自头足类生物碳酸盐的耗氧率和C-resp值之间的关系可能是结构和物种特异性的。我们的研究表明，新开发的代谢代理提供了一个有价值的替代方法来研究头足类的生理生态，进一步发展可能会扩大其适用性。
Measuring the metabolic rate of marine animals in their natural environment is challenging, impeding our understanding of their physiological ecology. Recently, a novel metabolic proxy, namely the delta C-13 values of biogenic carbonates (otoliths), was experimentally validated in teleost fishes. Cephalopods possess several types of biogenic carbonates, such as statolith, cuttlebone and other internal and external shells, which are all potential metabolic recorders, but few have been evaluated.To test the feasibility of the delta C-13 metabolic proxy in cephalopods, we conducted a temperature-controlled experiment with the pharaoh cuttlefish Sepia pharaonis to assess whether the proportion of metabolically derived carbon (C-resp) incorporated into statoliths and cuttlebones increases with metabolism in a warm environment. Moreover, we conducted multiple-species analysis using the published delta C-13 values of various biogenic carbonates to evaluate the consistency of the proxy among cephalopod species and between cephalopods and teleost fishes.In the temperature-controlled laboratory experiment, C-resp values calculated from statoliths and cuttlebones increased with an increase in environmental temperature. C-resp values did not differ between statoliths and cuttlebones. Moreover, the C-resp values of cephalopod biogenic carbonates had a similar range to those of fish otoliths and were correlated with metabolism-related factors, such as ambient temperature, body mass, ontogeny and functional behaviours, strengthening the feasibility of their use.The delta C-13 metabolic proxy reflects the total energy use in the natural environment, and it can be transformed into the oxygen consumption rate for a broader comparison with other species and for further evaluation with current theories related to metabolic ecology. However, experimental validation is highly recommended because the relationship between the oxygen consumption rate and C-resp values derived from biogenic carbonates of cephalopods might be structure- and species-specific. Our study revealed that the newly developed metabolic proxy provides a valuable alternative approach to study ecophysiology in cephalopods, and further development might broaden its applicability.