Characteristics of muscle fibers reconstituted in the regeneration process of masseter muscle in an mdx mouse model of muscular dystrophy
Characteristics of muscle fibers reconstituted in the regeneration process of masseter muscle in an mdx mouse model of muscular dystrophy
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DOI：
10.1007/s10974-006-9066-5
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发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Ide, Yoshinobu
中科院分区：
文献类型：
--
作者：
Lee, Won-Hyung;Abe, Shinichi;Ide, Yoshinobu
缺乏dystrophin的mdx小鼠在形态、蛋白质和转录水平上研究了咬肌生长过程中肌纤维特性的变化。在蛋白质和转录水平上检测了慢型亚型MyHC-1和快型亚型MyHC-2a、MyHC-2d和MyHC-2b。形态学检查显示，MDX小鼠咬肌组织发生变性、坏死和再生，尤以4周龄最为明显，9周龄时可见大量再生的肌纤维，细胞核居中。蛋白质和转录水平的检测结果表明，在肌肉纤维变性、坏死和再生的过程中，MDX小鼠咬肌获得了与正常小鼠咬肌完全不同的肌纤维特征。特别是在正常小鼠中很少发现的MyHC-1基因表达非常强烈。
Mdx mice, which lack dystrophin, were examined for changes in the properties of muscle fibers in the growth process of the masseter muscle at the morphological, protein and transcriptional levels. The slow-type isoform, MyHC-1, and the fast-type isoforms, MyHC-2a, MyHC-2d and MyHC-2b, were examined at the protein and the transcriptional level. Morphological examination showed that in the mdx mouse masseter muscle, degeneration, necrosis, and regeneration occurred, particularly at the age of 4 weeks, and many regenerated muscle fibers with centrally located nuclei were observed at the age of 9 weeks. The results of examination at the protein and the transcriptional level showed that in the process of muscle fiber degeneration, necrosis, and regeneration, the mdx mouse masseter muscle acquires muscle fiber characteristics entirely different from those in the normal mouse masseter muscle. In particular, MyHC-1, which is rarely found in normal mice, was very strongly expressed.