Characterization of 24 additional microsatellite loci in Spartina species (Poaceae)
Characterization of 24 additional microsatellite loci in Spartina species (Poaceae)
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DOI：
10.1007/s10592-005-9084-7
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发表时间：
2006-02
影响因子：
2.2
通讯作者：
Christina M. Sloop;Heather Mcgray;M. Blum;D. Strong
中科院分区：
文献类型：
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作者：
Christina M. Sloop;Heather Mcgray;M. Blum;D. Strong
在加利福尼亚州旧金山湾(SFB)，大西洋和墨西哥湾沿岸土生索麦草互花米草(Spartina Terniflora)和加州土生叶草草(S.foliosa)之间的入侵杂交种群正在以超过指数增长的速度传播(Ayres等人。2004年)。互花米草也以惊人的速度在华盛顿州的威拉帕湾(Willapa Bay，WB)传播。2005)，其中不发生叶状链霉菌。我们最初开发了11个微卫星基因座来阐明斯巴达入侵是如何进行的(Blum等人。2004年)。在这里，我们描述了另外24个微卫星座位的特征，以使更彻底的分子研究推动斯巴达纳入侵的生态和进化过程。所有新的基因座都是从http:///www.构建的针对(GA)n、(CA)n、(AAG)n和(CTA)n重复序列的四个丰富的基因组文库中开发出来的遗传服务。Com)从互花链霉菌基因组DNA(>；100lg)中分离自北卡罗来纳州的一株植物。根据地理信息系统聚合酶链式反应(PCR)文库筛选指南，我们发现大约40%(196个)的大肠杆菌克隆含有互花链霉菌的DNA片段。在ABI 3100测序仪(PE应用生物系统)上使用正向通用引物pUC19和ABI PRISM？BigDyeTM终止子化学方法对PCR产物进行测序。用3号引物(Rozen和Skaletsky，1998)和http://www.设计了152个克隆序列的位点特异性引物格拉梅尼。奥格
In San Francisco Bay (SFB), CA, an invasive hybrid swarm between the Atlantic and Gulf coast native cordgrass Spartina alterniflora and the California native S. foliosa, is spreading at a rate exceeding exponential growth (Ayres et al. 2004). Spartina alterniflora is also spreading at an extraordinary rate in Willapa Bay (WB), WA (Civille et al. 2005), where S. foliosa does not occur. We initially developed 11 microsatellite loci to elucidate how Spartina invasions proceed (Blum et al. 2004). Here we characterize an additional 24 microsatellite loci to enable more thorough molecular investigations of ecological and evolutionary processes driving Spartina invasions. All new loci were developed from four enriched genomic libraries targeting (GA) n,(CA) n,(AAG) n and (CTA) n repeat motifs constructed by Genetic Identification Services (GIS)(http:///www. geneticid-services. com) from S. alterniflora genomic DNA (> 100lg) isolated from a plant sampled from North Carolina. Following GIS polymerase chain reaction (PCR) library screening guidelines, we found that approximately 40%(196 of 500) of the E. coli clones had incorporated S. alternifloraDNA fragments. A forward universal pUC19 primer and ABI PRISMÒ BigDyeTM Terminator chemistry on an ABI 3100 sequencer (PE Applied Biosystems) were used to sequence the PCR products. Locus specific primers were designed for 152 clone sequences using PRIMER 3 (Rozen and Skaletsky 1998) and http://www. gramene. org