Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control
Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control
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DOI：
10.1101/gad.1603907
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发表时间：
2007-12-01
影响因子：
10.5
通讯作者：
Collins, Kathleen
中科院分区：
文献类型：
--
作者：
Hogg, J. Robert;Collins, Kathleen
人类表达四种不同的非蛋白编码Y RNA（ncRNA）。为了研究Y RNA的功能多样性，我们利用了一种基于RNA的亲和纯化方法来分离组装在单个人Y RNA上的核糖核蛋白（RNP）。银染和质谱分析显示，Ro和La蛋白与所有Y RNA组装，而其他蛋白与特定的Y RNA相关。出乎意料的是，Y 5 RNA独特地共纯化核糖体蛋白L5及其结合伴侣5S RNA。这些发现揭示了Y 5对5S监视的贡献，并表明Ro-Y 5和L5-5S RNP之间的相互作用将5S RNA确立为质量控制的靶标。
Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y RNAs. Unexpectedly, Y5 RNA uniquely copurified ribosomal protein L5 and its binding partner 5S RNA. These findings reveal a contribution of Y5 to 5S surveillance and suggest that interactions between Ro-Y5 and L5-5S RNPs establish 5S RNA as a target of quality control.