Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing
Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing
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通过 Solexa 测序对玉米大根早期发育过程中的 microRNA 进行转录谱分析
DOI：
10.1016/j.ygeno.2012.11.004
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发表时间：
2013-02-01
期刊：
影响因子：
4.4
通讯作者：
Zheng, Cheng-Chao
中科院分区：
文献类型：
--
作者：
Liu, Peng;Yan, Kang;Zheng, Cheng-Chao
为了鉴定与花托根发育有关的microRNAs，对3个文库进行了Solexa高通量测序，这些文库分别来自节点(N)、刚出苗的花托根(NR)和IAA处理(NRI)后刚出苗的花托根的节间。在N、NR和NRI文库中分别获得650,793,957,303和1,082,948个基因组匹配的唯一读数。进一步的分析证实了137个已知miRNAs的真实性，并在玉米中发现了159个新的miRNAs。利用qRT-PCR技术鉴定和验证了14个在玉米胚根发育过程中保守表达的miRNAs和16个差异表达的新miRNAs，以及15个目的基因。此外，我们在三个文库中鉴定了9个与miRNA前体匹配的可能形成miRNA簇的新sRNAs，以及24个ntsiRNAs。此外，我们认为生长素是根发育的调节因子，可以通过miRNAs在转录后水平上进行调节。(C)2012 Elsevier Inc.保留所有权利。
To characterize the microRNAs that contribute to the development of brace root, Solexa high-throughput sequencing of three libraries derived from tissues of node (N), nodes with just-emerged brace roots (NR), and nodes with just-emerged brace roots after IAA treatment (NRI) was performed. Total 650,793, 957,303 and 1,082,948 genome-matched unique reads were obtained in N, NR and NRI libraries, respectively. Further analysis confirmed the authenticity of 137 known miRNAs and the discovery of 159 novel miRNAs in maize. 14 conserved and 16 novel miRNAs differentially expressed in brace root, as well as 15 target genes, were identified and validated by qRT-PCR during maize brace root development. Moreover, we identified 9 miRNA precursor-matched novel sRNAs that may form miRNA clusters, as well as 24 nt siRNAs in the three libraries. In addition, we suggest that auxin represent a regulator in brace root development and can be regulated at the posttranscriptional level by miRNAs. (C) 2012 Elsevier Inc. All rights reserved.