Spatial light interference microscopy (SLIM).
Spatial light interference microscopy (SLIM).
复制标题
空间光干扰显微镜（Slim）。
DOI：
10.1364/oe.19.001016
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发表时间：
2011-01-17
期刊：
影响因子：
3.8
通讯作者：
Popescu G
中科院分区：
文献类型：
--
作者：
Wang Z;Millet L;Mir M;Ding H;Unarunotai S;Rogers J;Gillette MU;Popescu G
我们提出空间光干涉显微镜（SLIM）作为一种新的光学显微镜技术，能够测量纳米级的结构和动态活细胞通过干涉。SLIM结合了光成像中的两个经典概念：Zernike的相衬显微镜，它可以呈现透明样品的高对比度强度图像，以及Gabor的全息术，记录来自物体的相位信息。因此，SLIM揭示了细胞结构的内在对比度，此外，还提供了整个样品的定量光程长度图。由此产生的地形精度与原子力显微镜相当，而采集速度则高出1,000倍。我们说明了新的洞察细胞动力学通过SLIM从大鼠大脑的原代细胞培养的实验。SLIM是作为一个附加模块，以现有的相衬显微镜，这可能证明有助于在大规模的影响光学显微镜领域。
We present spatial light interference microscopy (SLIM) as a new optical microscopy technique, capable of measuring nanoscale structures and dynamics in live cells via interferometry. SLIM combines two classic ideas in light imaging: Zernike’s phase contrast microscopy, which renders high contrast intensity images of transparent specimens, and Gabor’s holography, where the phase information from the object is recorded. Thus, SLIM reveals the intrinsic contrast of cell structures and, in addition, renders quantitative optical path-length maps across the sample. The resulting topographic accuracy is comparable to that of atomic force microscopy, while the acquisition speed is 1,000 times higher. We illustrate the novel insight into cell dynamics via SLIM by experiments on primary cell cultures from the rat brain. SLIM is implemented as an add-on module to an existing phase contrast microscope, which may prove instrumental in impacting the light microscopy field at a large scale.