On the origin of branches in Escherichia coli
On the origin of branches in Escherichia coli
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DOI：
10.1128/jb.181.21.6607-6614.1999
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发表时间：
1999-11-01
影响因子：
3.2
通讯作者：
Nordström, K
中科院分区：
文献类型：
--
作者：
Gullbrand, B;Åkerlund, T;Nordström, K
一些大肠杆菌菌株，vith受损的细胞分裂形成分支细胞在某些生长条件下，在高频率，在这里，我们表明，无论是FtsI或FtsZ的活动是必需的分支的发展。芽没有形成在特定位置沿着细胞表面在高分支条件。影响细胞壁合成的抗生素在氨苄青霉素、头孢氨苄和青霉素G的情况下对分支形成有积极影响，而美西林和D-环丝氨酸没有实质性影响。改变细胞形态的营养变化表明，形态的变化之前分支，表明细胞的生理状态，而不是特定的介质成分诱导分支。最后，在具有芽或分支的细胞的子细胞中，芽形成的概率没有增加，表明芽形成是随机事件。我们认为，分支形成是由细胞壁伸长异常引起的，而不是由异常的细胞分裂事件引起的。
Some Escherichia coli strains,vith impaired cell division form branched cells at high frequencies during certain growth conditions, Here, we show that neither FtsI nor FtsZ activity is required for the development of branches. Buds did not form at specific positions along the cell surface during high-branching conditions. Antibiotics affecting cell wall synthesis had a positive effect on branch formation in the case of ampicillin, cephalexin, and penicillin G, whereas mecillinam and D-cycloserine had no substantial effect. Altering the cell morphology by nutritional shifts showed that changes in morphology preceded branching, indicating that the cell's physiological state rather than specific medium components induced branching. Finally, there was no increased probability for bud formation in the daughters of a cell with a bud or branch, showing that bud formation is a random event, We suggest that branch formation is caused by abnormalities in cell wall elongation rather than by aberrant cell division events.