In vitro propagation of Pimelea spicata R.Br (Thymelaeaceae), an endangered species of the Sydney region, Australia
In vitro propagation of Pimelea spicata R.Br (Thymelaeaceae), an endangered species of the Sydney region, Australia
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DOI：
10.1007/s11240-009-9534-x
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
C. Offord;J. Tyler
中科院分区：
文献类型：
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作者：
C. Offord;J. Tyler
微繁殖作为一种迁地保护策略进行了评估，澳大利亚濒危植物Pimelea spicata（百里香科）。虽然实现了该物种的再生，但观察和检查了一些生理问题。P. spicata在MS培养基上的增殖率高于在1/2 MS培养基上的增殖率，但在高盐培养基上的茎尖坏死率显著高于在高盐培养基上的茎尖坏死率。增加钙浓度和气体交换加剧茎尖坏死。在所有处理中均产生了许多过度水合的芽，其原因无法确定，尽管在1/2 MS处理中观察到较少的过度水合。在不存在植物生长调节剂的情况下，在1/2 MS上体外生根的芽成功地适应了温室条件，而使用IBA凝胶处理的微芽的直接生根被证明是不成功的。这是该濒危物种组织培养繁殖的首次报道。
Micropropagation was assessed as an ex situ conservation strategy for the endangered Australian plantPimelea spicata(Thymelaeaceae). Although regeneration of this species was achieved, several physiological problems were observed and examined. Explants ofP. spicatahad a higher multiplication rate on MS medium, than on ½ MS, but there was a significantly higher percentage of necrotic shoot tips on the higher salt medium. Increasing calcium concentration and gas exchange exacerbated shoot-tip necrosis. A number of hyperhydrated shoots were produced in all treatments, the cause of which could not be determined, although less hyperhydicity was observed in the ½ MS treatment. Shoots, rooted in vitro on ½ MS in the absence of plant growth regulators, were successfully acclimatised to greenhouse conditions, while direct rooting of microshoots using IBA gel treatment proved unsuccessful. This is the first report of tissue culture propagation of this endangered species.