Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis
Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis
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DOI：
10.1099/mic.0.29137-0
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发表时间：
2006-10-01
期刊：
影响因子：
2.8
通讯作者：
Siezen, Roland J.
中科院分区：
文献类型：
--
作者：
de Been, Mark;Francke, Christof;Siezen, Roland J.
蜡样芽孢杆菌组的成员普遍存在于环境中，并能适应大范围的环境波动。在细菌中，这些适应性反应通常由双组分信号转导系统（TCS）介导，所述双组分信号转导系统由组氨酸激酶（HK）及其同源反应调节因子（RR）组成。利用计算机模拟技术，从8个完全测序的B中回收了一套完整的HK和RR。cereus组基因组。通过应用双向最佳命中方法结合基因邻域分析，这些蛋白质的足迹。在B的每个成员中检测到大约40个HK-RR基因对。cereus群。此外，每个成员包含许多HK和RR基因不是成对编码的（“孤儿”）。分类的HK和RR的基础上，他们的酶结构域与两个相邻的连接树的分析，这些结构域揭示了推定的相互作用的合作伙伴，为大多数的“酶”。推测的生物学功能，包括参与毒力和宿主-微生物相互作用，预测了B。蜡样芽胞杆菌组HK和RR与B组比较。枯草杆菌和其他微生物。很明显，B。炭疽杆菌似乎缺乏特异性HK和RR，并发现含有许多截短的、无功能的HK和RR基因。假设B.炭疽菌作为病原体可能减少了它所暴露的环境刺激的范围。这可能使它的一些TCS过时，最终导致一些HK和RR基因的缺失。
Members of the Bacillus cereus group are ubiquitously present in the environment and can adapt to a wide range of environmental fluctuations. In bacteria, these adaptive responses are generally mediated by two-component signal transduction systems (TCSs), which consist of a histidine kinase (HK) and its cognate response regulator (RR). With the use of in silico techniques, a complete set of HKs and RRs was recovered from eight completely sequenced B. cereus group genomes. By applying a bidirectional best-hits method combined with gene neighbourhood analysis, a footprint of these proteins was made. Around 40 HK-RR gene pairs were detected in each member of the B. cereus group. In addition, each member contained many HK and RR genes not encoded in pairs ('orphans'). Classification of HKs and RRs based on their enzymic domains together with the analysis of two neighbour-joining trees of these domains revealed putative interaction partners for most of the 'orphans'. Putative biological functions, including involvement in virulence and host-microbe interactions, were predicted for the B. cereus group HKs and RRs by comparing them with those of B. subtilis and other micro-organ isms. Remarkably, B. anthracis appeared to lack specific HKs and RRs and was found to contain many truncated, putatively nonfunctional, HK and RR genes. It is hypothesized that specialization of B. anthracis as a pathogen could have reduced the range of environmental stimuli to which it is exposed. This may have rendered some of its TCSs obsolete, ultimately resulting in the deletion of some HK and RR genes.