GONADAL STEROIDOGENESIS IN-VITRO FROM JUVENILE ALLIGATORS OBTAINED FROM CONTAMINATED OR CONTROL LAKES
GONADAL STEROIDOGENESIS IN-VITRO FROM JUVENILE ALLIGATORS OBTAINED FROM CONTAMINATED OR CONTROL LAKES
复制标题
DOI：
10.2307/3432409
复制
发表时间：
1995-05-01
影响因子：
10.4
通讯作者：
PERCIVAL, HF
中科院分区：
文献类型：
--
作者：
GUILLETTE, LJ;GROSS, TS;PERCIVAL, HF
许多污染物的普遍分布以及这些污染物对生殖、内分泌和免疫系统的非致命性、多代影响引起了人们对全世界野生动物受到影响的担忧。尽管某些物种的致病因素和影响是已知的，但与污染物引起的生殖改变相关的潜在生理机制仍然知之甚少，需要进行广泛的研究。我们描述了一项研究，检查从佛罗里达州中部受污染或控制湖泊获得的幼年短吻鳄（密西西比短吻鳄）身上取出的性腺的类固醇生成活性。当体外比较受污染湖泊和对照湖泊的卵巢时，雌二醇-17β (E(2)) 的合成存在显着差异。此外，与对照雄性的睾丸相比，从受污染的阿波普卡湖获得的雄性睾丸合成的 E(2) 浓度明显更高。相比之下，本研究中检查的所有睾丸的睾酮（T）合成显示出正常模式，并且产生的浓度高于从任一湖获得的卵巢中观察到的浓度。有趣的是，性腺类固醇生成的模式与之前报道的从同一个体获得的这些激素的血浆浓度不同。我们认为体内和体外模式之间的差异是由于血浆性类固醇激素的肝脏降解的改变造成的。
The ubiquitous distribution of many contaminants and the nonlethal, multigenerational effects of such contaminants on reproductive, endocrine, and immune systems have led to concerns that wildlife worldwide are affected. Although the causal agents and effects are known for some species, the underlying physiological mechanisms associated with contaminant-induced reproductive modifications are still poorly understood and require extensive research. We describe a study examining the steroidogenic activity of gonads removed from juvenile alligators (Alligator mississippiensis) obtained from contaminated or control lakes in central Florida. Synthesis of estradiol-17 beta (E(2)) was significantly different when ovaries from the contaminated and control lakes were compared in vitro. Additionally, testes from males obtained from the contaminated lake, Lake Apopka, synthesized significantly higher concentrations of E(2) when compared to testes obtained from control males. In contrast, testosterone (T) synthesis from ail testes examined in this study displayed a normal pattern and produced concentrations greater than that observed from ovaries obtained from either lake. Interestingly, the pattern of gonadal steroidogenesis differs from previously reported plasma concentrations of these hormones obtained from the same individuals. We suggest that the differences between the in vivo and in vitro patterns are due to modifications in the hepatic degradation of plasma sex steroid hormones.