Effects of modified Shoutaiwai recipe on integrin β3 and leukemia-inhibitory factor in endometrium of controlled ovarian hyperstimulation mice during the implantation window
Effects of modified Shoutaiwai recipe on integrin β3 and leukemia-inhibitory factor in endometrium of controlled ovarian hyperstimulation mice during the implantation window
复制标题
DOI：
10.4238/2015.april.10.6
复制
发表时间：
2015-01-01
影响因子：
0.4
通讯作者：
Wu, X. H.
中科院分区：
文献类型：
--
作者：
Chen, X. Y.;Chen, J.;Wu, X. H.
本文研究了寿台外方对控制性卵巢过度刺激（COH）小鼠着床窗期子宫内膜整合素β 3和白血病抑制因子（LIF）的影响。将70只未怀孕小鼠随机分为3组：中药治疗组（N = 30）、阿司匹林治疗组（N = 30）和对照组（N = 10）。造模成功后，给药组和对照组小鼠分别加味寿台外方、阿司匹林、0.9%生理盐水。在小鼠着床窗口期，恢复小鼠子宫中段，分别采用免疫组织学两步法和逆转录- pcr检测子宫内膜中整合素β 3和LIF的表达。中药治疗组小鼠子宫内膜整合素β 3和LIF的表达明显高于阿司匹林治疗组和对照组，阿司匹林治疗组小鼠子宫内膜整合素β 3和LIF的表达明显高于对照组。我们改良的守台外方可能通过增加着床窗口期子宫内膜中整合素β 3和LIF的表达来改善COH小鼠子宫内膜的可接受性。
We investigated the effects of a modified Shoutaiwai recipe on integrin beta 3 and leukemia-inhibitory factor (LIF) in the endometrium of controlled ovarian hyperstimulation (COH) mice during the implantation window. Seventy non-pregnant mice were randomly divided into 3 groups: a traditional medicine (TCM) treatment group (N = 30), an aspirin treatment (N = 30) group, and a control group (N = 10). After the model was successfully established, mice in the drug treatment groups and the control group were respectively treated with the modified Shoutaiwai recipe, aspirin, or 0.9% physiological saline. During the implantation window of mice, the middle segment of the mouse uterus was recovered, and integrin beta 3 and LIF expressions in the endometrium were respectively detected using an immunohistological two-step method and reverse transcription-PCR. Expressions of integrin beta 3 and LIF in the endometrium of mice in the TCM treatment group were significantly increased compared to aspirin-treated and control mice, and those of aspirin-treated mice were increased compared to the control group. Our modified Shoutaiwai recipe may improve the endometrial receptivity of COH mice by increasing the expression of integrin beta 3 and LIF in the endometrium during the implantation window.