The role of NK cells in antitumor activity of dietary fucoidan from Undoria pinnotifida sporophylls (Mekabu)
The role of NK cells in antitumor activity of dietary fucoidan from Undoria pinnotifida sporophylls (Mekabu)
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DOI：
10.1055/s-2006-951703
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发表时间：
2006-12-01
期刊：
影响因子：
2.7
通讯作者：
Nakano, Takahisa
中科院分区：
文献类型：
--
作者：
Maruyama, Hiroko;Tamauchi, Hidekazu;Nakano, Takahisa
褐藻糖胶是裙带菜的孢子叶，其抗肿瘤活性可能与增强免疫反应有关。本报告描述了膳食Mekabu褐藻糖胶对小鼠A20白血病细胞肿瘤生长和T细胞受体转基因（DO-11-10-Tg）小鼠T细胞介导的免疫应答的影响。用含有1% Mekabu岩藻依聚糖（0.034 +/- 0.003 g/小鼠/天）的饮食喂养动物10天，并皮下（s.c.）接种A20白血病细胞。此后，用含有岩藻依聚糖的饮食喂养小鼠40天。岩藻依聚糖硫酸酯对肿瘤的抑制率为65.4%。我们研究了如何T细胞介导的和自然杀伤（NK）细胞的杀伤活性增强DO-11-10小鼠喂食Mekabu褐藻糖胶。与基础饮食相比，在喂食岩藻多糖的小鼠中，卵清蛋白（OVA）（其对OVA转染的A20（OVA-A20）B淋巴瘤细胞具有特异性）和NK细胞对YAC-1的细胞溶解活性显著增强。因此，这些发现表明，Mekabu褐藻糖胶通过Th 1细胞和NK细胞应答介导肿瘤破坏。
Fucoidan from Mekabu (sporophyll of Undaria pinnatifida), a dietary alga, exerts antitumor activity possibly through enhancing the immune response. The present report describes the effects of dietary Mekabu fucoidan on the tumor growth of mouse A20 leukemia cells and on T cell-mediated immune responses in T cell receptor transgenic (DO-11-10-Tg) mice. The animals were fed with a diet containing 1% Mekabu fucoidan (0.034 +/- 0.003 g/mouse/day) for 10 days and subcutaneously (s.c.) inoculated with A20 leukemia cells. Thereafter, the mice were fed with the diet containing fucoidan for 40 days. Mekabu fucoidan inhibited tumors by 65.4%. We studied how the killer activities of T cell-mediated and natural killer (NK) cells are augmented in DO-11-10 mice fed with Mekabu fucoidan. The cytolytic activities of ovalbumin (OVA), which is specific against OVA-transfected A20 (OVA-A20) B lymphoma cells, and NK cells against YAC-1 were significantly enhanced in the mice fed with fucoiclan compared with a basic diet. Thus, these findings suggested that Mekabu fucoidan mediates tumor destruction through Th1 cell and NK cell responses.