pDNA-loaded Bubble liposomes as potential ultrasound imaging and gene delivery agents
pDNA-loaded Bubble liposomes as potential ultrasound imaging and gene delivery agents
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DOI：
10.1016/j.biomaterials.2012.12.018
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发表时间：
2013-04-01
期刊：
影响因子：
14
通讯作者：
Aramaki, Yukihiko
中科院分区：
文献类型：
--
作者：
Endo-Takahashi, Yoko;Negishi, Yoichi;Aramaki, Yukihiko
我们已经开发了聚乙二醇修饰脂质体（气泡脂质体；BLs），它可以捕获超声造影剂气体，并且我们已经报道了BLs和US暴露的组合是体外和体内递送pDNA和siRNA的有效工具。在本研究中，我们使用三种阳离子脂质制备了pdna负载的BLs，通过全身注射来提高US成像效果和转染效率。我们研究了这些BLs的US成像能力，它们对血清成分pDNA的保护作用，以及它们在体外和体内的转染效果。因此，我们证明了US成像能力和转染效果随脂质成分的不同而变化，并且含有DSDAP的p-BLs可能是三种类型的p-BLs中最稳定和有效的工具。事实上，在缺血肌肉中，含有DSDAP的p-BLs可以通过诊断性US检测到，并且可以通过治疗性US传递表达bfgf的pDNA，从而诱导血管生成因子和改善血流。这些结果表明，将p-BLs与US暴露相结合可能有助于稳定的US成像和有效的基因传递，并可能导致建立一种疾病诊断和治疗相结合的治疗方法。(c) 2013 Elsevier Ltd.版权所有。
We have developed polyethyleneglycol (PEG)-modified liposomes (Bubble liposomes; BLs) that entrap ultrasound (US) contrast gas, and we have reported that the combination of BLs and US exposure was an effective tool for delivering pDNA and siRNA in vitro and in vivo. In this study, we prepared pDNA-loaded BLs using three types of cationic lipids to enhance the US imaging effect and the transfection efficiency via systemic injection. We investigated the US imaging abilities of these BLs, their protective effects on pDNA from serum component, and their transfection effects in vitro and in vivo. As a result, we demonstrated that the US imaging ability and transfection effect varied with lipid component and that p-BLs containing DSDAP could be the most stable and effective tool the among three types of p-BLs. Indeed, in ischemic muscle, p-BLs containing DSDAP could be detected using diagnostic US and could deliver bFGF-expressing pDNA using therapeutic US, leading to the induction of angiogenic factors and the improvement of blood flow. These results suggest that combining p-BLs with US exposure may be useful for stable US imaging and efficient gene delivery and may lead to the establishment of a theranostic approach, which is a combination of disease diagnosis and therapy. (c) 2013 Elsevier Ltd. All rights reserved.