Increasing Prevalence of ESBL-Producing Multidrug Resistance Escherichia coli From Diseased Pets in Beijing, China From 2012 to 2017
Increasing Prevalence of ESBL-Producing Multidrug Resistance Escherichia coli From Diseased Pets in Beijing, China From 2012 to 2017
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DOI：
10.3389/fmicb.2019.02852
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发表时间：
2019-12-10
影响因子：
5.2
通讯作者：
Xia, Zhaofei
中科院分区：
文献类型：
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作者：
Chen, Yanyun;Liu, Zhihai;Xia, Zhaofei
我们调查了从宠物中分离的产超广谱β-内酰胺酶的大肠埃希菌的耐药性趋势和特征，以及这是否与临床使用抗生素有关。对含有病猫和狗的大肠杆菌的临床样本进行了抗生素敏感性和相关的基因特征筛查。我们从来自狗(n=1565)和猫(n=321)的1886份标本中鉴定出127株大肠埃希菌，其中大部分来自尿路感染(n=108，85%)。对β-内酰胺类和氟喹诺酮类的耐药率较高，对3类抗生素的耐药率从2012年的67%上升到2017年的75%(P<；0.0001)。尤其是对6-9种抗生素耐药的菌株，从26.67%上升到60.71%。随着耐药率的增加，临床治疗中使用β-内酰胺类药物的比例也增加了。因此，最常见的亚型是bla(CTX-M)(n=44，34.65%)、bla(CTX-M-65)(n=19)、bla(CTX-M-15)(n=18)和qnrB(n=119，93.70%)。BLA(CTX-M)分离株有36种独特的脉冲场电泳型(PFGE)和28种不同的序列类型(STS)，其中ST405(7，15.9%)、ST131(3，6.8%)、ST73、ST101、ST372和ST827(2，4.5%)是最常见的。这一数据表明，BLA(CTX-M)阳性的大肠杆菌分离株具有高度的多样性。在2株ST101株和1株ST405株中检出BLA(NDM-5)3株(2.36%)，在3株具有3种不同STS(ST6316、ST405和ST46)的粘菌素耐药大肠杆菌中也检出mcr-1。我们的研究表明，耐多药和产超广谱β-内酰胺酶的大肠埃希菌有增加的趋势，这与使用β-内酰胺类抗生素治疗这些动物有关。这一数据表明，耐药细菌从宠物传播到人类的风险很大，宠物使用抗生素应该得到更严格的监管。
We investigated antimicrobial resistance trends and characteristics of ESBL-producing Escherichia coli isolates from pets and whether this correlates with antibiotic usage in the clinic. Clinical samples containing E. coli from diseased cats and dogs were screened for antibiotic sensitivity and associated genotypic features. We identified 127 E. coli isolates from 1886 samples from dogs (n = 1565) and cats (n = 321) with the majority from urinary tract infections (n = 108, 85%). High rates of resistance were observed for beta-lactams and fluoroquinolones and resistance to > 3 antibiotic classes (MDR) increased from 67% in 2012 to 75% in 2017 (P < 0.0001). This was especially true for strains resistant to 6-9 antibiotics that increased from 26.67 to 60.71%. Increased rates in beta-lactam use for clinical treatment accompanied these increasing resistance rates. Accordingly, the most frequently encountered subtypes were bla(CTX-M) (n = 44, 34.65%), bla(CTX-M-65) (n = 19) and bla(CTX-M-15) (n = 18) and qnrB (n = 119, 93.70%). The bla(CTX-M)-isolates possessed 36 unique pulsed field electrophoretic types (PFGEs) and 28 different sequence types (STs) in ST405 (7, 15.9%), ST131 (3, 6.8%), ST73, ST101, ST372, and ST827 (2, 4.5% each) were the most prevalent. This data demonstrated a high level of diversity for the bla(CTX-M)-positive E. coli isolates. Additionally, bla(NDM-5) was detected in three isolates (n = 3, 2.36%), comprised of two ST101 and one ST405 isolates, and mcr-1 was also observed in three colistin-resistant E. coli with three different STs (ST6316, ST405, and ST46). Our study demonstrates an increasing trend in MDR and ESBL-producing E. coli and this correlated with beta-lactam antibiotic usage for treatment of these animals. This data indicates that there is significant risk for the spread of resistant bacteria from pets to humans and antibiotic use for pets should be more strictly regulated.