Electrospun Polycaprolactone 3D Nanofibrous Scaffold with Interconnected and Hierarchically Structured Pores for Bone Tissue Engineering
Electrospun Polycaprolactone 3D Nanofibrous Scaffold with Interconnected and Hierarchically Structured Pores for Bone Tissue Engineering
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DOI：
10.1002/adhm.201500345
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发表时间：
2015-10
影响因子：
10
通讯作者：
Tao Xu;Jacob M. Miszuk;Yong Zhao;Hongli Sun;H. Fong
中科院分区：
文献类型：
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作者：
Tao Xu;Jacob M. Miszuk;Yong Zhao;Hongli Sun;H. Fong
第一次，电纺聚己内酯（PCL）3D纳米纤维支架已经通过创新和方便的方法（即，热诱导自聚集，然后冷冻干燥），并且支架具有互连的和分级结构的孔，包括尺寸高达300 μm的大孔。新型PCL 3D支架柔软而有弹性，孔隙率高达96.4%，因此在形态/结构上与天然细胞外基质相似，非常适合细胞功能和组织形成。体外研究表明，该支架可以导致高细胞活力;更重要的是，它能够促进BMP 2诱导的小鼠骨髓间充质干细胞的成软骨分化比成骨分化更有效。与体外研究结果一致，体内结果表明，电纺PCL 3D支架通过生理性软骨内骨化过程作为功能性骨再生的有利合成细胞外基质。
For the first time, electrospun polycaprolactone (PCL) 3D nanofibrous scaffold has been developed by an innovative and convenient approach (i.e., thermally induced nanofiber self‐agglomeration followed by freeze drying), and the scaffold possesses interconnected and hierarchically structured pores including macropores with sizes up to ≈300 μm. The novel PCL 3D scaffold is soft and elastic with very high porosity of ≈96.4%, thus it is morphologically/structurally similar to natural extracellular matrix and well suited for cell functions and tissue formation. The in vitro studies reveal that the scaffold can lead to high cell viability; more importantly, it is able to promote more potent BMP2‐induced chondrogenic than osteogenic differentiation of mouse bone marrow mesenchymal stem cells. Consistent to the in vitro findings, the in vivo results indicate that the electrospun PCL 3D scaffold acts as a favorable synthetic extracellular matrix for functional bone regeneration through the physiological endochondral ossification process.