Pinhole micro-SPECT/CT for noninvasive monitoring and quantitation of oncolytic virus dispersion and percent infection in solid tumors.
Pinhole micro-SPECT/CT for noninvasive monitoring and quantitation of oncolytic virus dispersion and percent infection in solid tumors.
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作者：
The purpose of our study was to validate the ability of pinhole micro-single-photon emission computed tomography/computed tomography (SPECT/CT) to 1) accurately resolve the intratumoral dispersion pattern and 2) quantify the infection percentage in solid tumors of an oncolytic measles virus encoding the human sodium iodide symporter (MV-NIS). NIS RNA level and dispersion pattern were determined in control and MV-NIS infected BxPC-3 pancreatic tumor cells and mouse xenografts using quantitative, real-time, reverse transcriptase, polymerase chain reaction, autoradiography, and immunohistochemistry (IHC). Mice with BxPC-3 xenografts were imaged with 123I or 99TcO4 micro-SPECT/CT. Tumor dimensions and radionuclide localization were determined with imaging software. Linear regression and correlation analyses were performed to determine the relationship between tumor infection percentage and radionuclide uptake (% injected dose per gram) above background and a highly significant correlation was observed (r2 = 0.947). A detection threshold of 1.5-fold above the control tumor uptake (background) yielded a sensitivity of 2.7% MV-NIS infected tumor cells. We reliably resolved multiple distinct intratumoral zones of infection from noninfected regions. Pinhole micro-SPECT/CT imaging using the NIS reporter demonstrated precise localization and quantitation of oncolytic MV-NIS infection and can replace more time-consuming and expensive analyses (eg, autoradiography and IHC) that require animal sacrifice.
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影响因子：
12.4
作者：
Barton, KN;Tyson, D;Brown, SL
通讯作者：
Brown, SL
DOI：
10.1007/s00259-004-1715-6
发表时间：
2005-04-01
影响因子：
9.1
作者：
Niu, G;Krager, KJ;Domann, FE
通讯作者：
Domann, FE
影响因子：
11.5
作者：
Hasegawa, K;Pham, L;Peng, KW
通讯作者：
Peng, KW
影响因子：
7.4
作者：
Haddad D;Chen NG;Zhang Q;Chen CH;Yu YA;Gonzalez L;Carpenter SG;Carson J;Au J;Mittra A;Gonen M;Zanzonico PB;Fong Y;Szalay AA
通讯作者：
Szalay AA
影响因子：
6.7
作者：
Myers, R. M.;Greiner, S. M.;Russell, S. J.
通讯作者：
Russell, S. J.