Transcriptional collision between convergent genes in budding yeast
Transcriptional collision between convergent genes in budding yeast
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DOI：
10.1073/pnas.132270899
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发表时间：
2002-06-25
影响因子：
11.1
通讯作者：
Proudfoot, NJ
中科院分区：
文献类型：
--
作者：
Prescott, EM;Proudfoot, NJ
简单的真核生物如酿酒酵母的基因和调控元件之间的转录干扰是其压缩的遗传排列的不可避免的结果。我们以前已经表明，与串联排列的基因GAL10和GAL7，无效的上游基因的转录终止抑制下游基因的转录起始。我们现在表明，转录干扰也可以发生与S。酿酒酵母RNA聚合酶11个基因的排列呈会聚性。我们证明，当GAL10和GAL7基因在一个收敛的方向重排，转录起始发生在全水平。然而，一旦两个转录本开始重叠，延伸就受到限制，导致稳态mRNA积累严重减少。这种效应仅在顺式排列中观察到，反对RNA干扰作用于反义转录物的潜在产生。这些数据加强了通过有效终止信号分离相邻RNA聚合酶11转录单位的必要性。
Transcriptional interference between genes and the regulatory elements of simple eukaryotes such as Saccharomyces cerevisiae is an unavoidable consequence of their compressed genetic arrangement. We have shown previously that with the tandem arranged genes GAL10 and GAL7, inefficient transcriptional termination of the upstream gene inhibits initiation of transcription on the downstream gene. We now show that transcriptional interference can occur also with S. cerevisiae RNA polymerase 11 genes arranged convergently. We demonstrate that when the GAL10 and GAL7 genes are rearranged in a convergent orientation, transcriptional initiation occurs at full levels. However, as soon as the two transcripts begin to overlap, elongation is restricted, resulting in a severe reduction in steady-state mRNA accumulation. This effect is observed only in cis arrangement, arguing against RNA-interference effects acting on the potential generation of antisense transcripts. These data reinforce the necessity of separating adjacent RNA polymerase 11 transcription units by efficient termination signals.