Hypertrophic osteopathy associated with ovarian carcinoma in a mare
Hypertrophic osteopathy associated with ovarian carcinoma in a mare
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与母马卵巢癌相关的肥大性骨病
DOI：
10.1136/vr.143.6.172
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
J. Koeman
中科院分区：
文献类型：
--
作者：
H. J.;van;der Kolk;S. Geelen;H. F.;Jonker;J. Pycock;J. Koeman
第1次访视（rs=0-63，P<0.001）、第2次访视（rs=0.74，P<0.001）以及两次访视的评分和计数合并时（rs=0*69，P<0.001）使用拭子法进行细菌生长。清洁剂擦洗计数和半定量拭子技术之间的良好相关性表明，后一种方法可用于评估犬皮肤细菌种群密度，以进行比较，但并非绝对目的。该技术可用于快速、现场处理洗涤剂擦洗样本不切实际的情况，以及首选将拭子运送到细菌学实验室的情况。拭子技术有几个缺点。洗涤剂擦洗法可得出种群密度，而拭子法只能用于半定量评估。诸如擦拭皮肤时使用的压力以及任何渗出物的性质和程度的变化等因素预计会影响皮肤微生物的回收。在微生物从皮肤转移到拭子、从拭子转移到琼脂以及随后通过接种环将接种物扩散到培养基表面的过程中，可能会发生进一步的变异性。因此，通过拭子技术获得的结果只能用作种群密度的指南，指示一般趋势而不是绝对微生物计数。洗涤剂擦洗样品可以接种到不同的培养基上，并且很容易获得不同种类的细菌和酵母菌的计数。使用样品的连续稀释液可确保分离的菌落能够被识别和计数。相反，当需要计数不同菌种时，拭子技术不适用，因为通常会获得融合生长。然而，如果采样的皮肤区域主要由一种细菌定殖或感染，如犬化脓性皮炎的常见情况，则这可能不太重要。这项研究中描述的拭子技术应该证明是有用的
for bacterial growth using the swab method at visit one (rs=0-63, P<0.001), visit two (rs=0.74, P<0.001), and when the scores and counts at both visits were combined (rs=0*69, P<0001). The good correlation between the detergent scrub counts and the semi-quantitative swab technique indicates that the latter method can be used to assess cutaneous bacterial population densities in dogs for comparative but not absolute purposes. The technique could be used in situations where rapid, on-site processing of detergent scrub samples is impractical and where transport of the swab to a bacteriological laboratory is preferred. The swab technique has several disadvantages. The detergent scrub method yields a population density whereas the swab method can only be used to give a semi-quantitative assessment. Factors such as the pressure used on swabbing the skin and variation in the nature and degree of any exudate would be expected to influence the recovery of microbes from the skin. Further variability may occur in the transfer of organisms from the skin to the swab, from the swab to the agar and subsequent spread of inoculum by the loop over the surface of the culture medium. Thus results obtained by the swab technique can only be used as a guide to population densities, indicating general trends rather than absolute microbial counts. Detergent scrub samples can be inoculated onto different media and counts of different species of bacteria and yeasts are readily obtained. The use of serial dilutions of the sample ensures that isolated colonies can be identified and counted. In contrast, the swab technique is not appropriate when counts of different species are needed because confluent growth is often obtained. However, if the skin areas sampled are colonised or infected predominantly by one species of bacterium, as is often the case in canine pyoderma, then this may be of lesser importance. The swab technique described in this study should prove useful