Formation of in vivo tissue engineered human hyaline cartilage in the shape of a trachea with internal support
Formation of in vivo tissue engineered human hyaline cartilage in the shape of a trachea with internal support
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DOI：
10.1016/j.ijporl.2005.04.026
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发表时间：
2005-11-01
影响因子：
1.5
通讯作者：
Bin Saim, A
中科院分区：
文献类型：
--
作者：
Idrus, RBH;Chua, K;Bin Saim, A
目的：先天性和创伤后气管狭窄的治疗和管理仍然是小儿外科的一个挑战。本研究的目的是通过使用可生物降解的水凝胶和不可生物降解的高密度聚乙烯（HDP）的组合作为内部预定形状scaffolds.Methods：人鼻中隔软骨作为多余的组织后，选择性鼻中隔成形术和0.6%的胶原酶II消化收获与人鼻中隔软骨细胞重建气管。将软骨细胞培养在Ham’s F12培养基和Dulbecco’s改良Eagle培养基的等体积混合物中，该混合物添加有10%胎牛血清和碱性成纤维细胞生长因子。两次传代后，将培养的软骨细胞胰蛋白酶消化并与可生物降解的水凝胶Pluronic F127混合。然后将软骨细胞-水凝胶混合物涂在HDP上作为预定气管形状的内部支持物。结果：体内植入8周后，获得组织工程气管。组织工程化气管构建物的宏观外观表明，HDP 80-90%覆盖有淡黄色发亮的软骨样组织，没有任何炎症迹象。组织工程化气管软骨由均匀分布的陷窝组成，包埋在嗜碱性基质中，番红O染色显示大量蛋白多糖产生。结论：以人鼻中隔软骨细胞为支架，HDP为内支架，成功构建了在体气管。该结构具有生物惰性和强度的优点，这两者都是气管中的重要特性。重建(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Objective: Treatment and management of congenital as well as post-traumatic trachea stenosis remains a challenge in pediatric surgery. The aim of this study was to reconstruct a trachea with human nasal septum chondrocytes by using the combination of biodegradable hydrogel and non-biodegradable high-density polyethylene (HDP) as the internal predetermined shape scaffold.Methods: Human nasal septum cartilage was harvested as excessive tissue after elective septoplasty and digested in 0.6% collagenase II. Chondrocytes were cultured in an equal volume mix of Ham's F12 medium and Dulbecco's modified eagle medium added with 10% fetal bovine serum and basic fibroblast growth factor. After two passages, the cultured chondrocytes were trypsinized and mixed with biodegradable hydrogel Pluronic F127. The chondrocytes-hydrogel admixture was then painted over the HDP as the internal support in a predetermined trachea shape. The composite was then implanted subcutaneously in athymic mice.Results: After 8 weeks of in vivo implantation, the tissue engineered trachea constructs were harvested. Macroscopic appearance of the tissue engineered trachea constructs demonstrated that the HDP were 80-90% covered with yellowish glistering cartilage like tissue without any sign of inflammation. The tissue engineered trachea cartilage consisted of evenly spaced lacunae embedded in basophilic matrix and stained red with Safranin-O staining denoting abundant proteoglycans production. Type II collagen gene which was expressed in native cartilage was highly expressed in this tissue engineered trachea cartilage.Conclusion: We have successfully reconstructed a trachea in vivo with human nasal septum chondrocytes using HDP as the internal support. This construct has the advantage of bio-inert and strength in which both are important properties in tracheal. reconstruction. (c) 2005 Elsevier Ireland Ltd. All rights reserved.