First Report of Genotyping of Toxoplasma gondii Isolates From Wild Birds in China
First Report of Genotyping of Toxoplasma gondii Isolates From Wild Birds in China
复制标题
中国野生鸟类弓形虫基因分型首次报告
DOI：
10.1645/ge-3038.1
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发表时间：
2012-06-01
影响因子：
1.3
通讯作者：
Zhu, Xing-Quan
中科院分区：
文献类型：
--
作者：
Huang, Si-Yang;Cong, Wei;Zhu, Xing-Quan
摘要：弓形虫是一种重要的世界性机会寄生原虫，严重威胁着人类和动物的健康。来自南美洲的分离株的遗传特征显示出高度的遗传多样性。相比之下，来自北美和欧洲的分离株是高度克隆的，具有3个主要谱系，称为I型、II型和III型。然而，有限的资料，T。中华人民共和国已报道了弓形虫基因型。本研究对我国野生鸟类中的寄生虫进行了遗传多样性调查。对98只雉鸡（Phasianus colchicus）、35只树麻雀（Passer montanus）、22只家麻雀（Passer arteriticus）、20只沙麻雀（Passer ammocalus）和1只褐家雀（Passer rutilans）共178只野生鸟类的胸肌组织进行了T.弓形虫感染，其中4例弓形虫阳性。PCR扩增弓形虫B1基因。这些阳性DNA样本在10个遗传标记处进行了分型，其中包括9个核基因座，即，SAG 1、5′-和3′-SAG 2、备选SAG 2、SAG 3、GRA 6、L358、PK 1、c22-8、c29-2和顶质体基因座Apico。其中，3个分离株进行了基因分型，所有位点的完整数据，并确定了2种基因型（I型和II型变异）。这是首次报道T.我国不同地区野鸟弓形虫分离株的分布。结果表明，我国野生鸟类中存在I型和II型变异株，这些鸟类是T.弓形虫传播
Abstract: Toxoplasma gondii is an important cosmopolitan opportunistic protozoan parasite, which threatens the health of human beings and animals. Genetic characterization of isolates from South America has revealed high genetic diversity. In contrast, isolates from North America and Europe were highly clonal, with 3 major lineages known as the Types I, II, and III. However, limited information on T. gondii genotypes has been reported in The People's Republic of China. Here we conducted a survey to determine genetic diversity of this parasite in wild birds of China. In total, tissues from breast muscle of 178 wild birds, including 98 common pheasants (Phasianus colchicus), 35 tree sparrows (Passer montanus), 22 house sparrows (Passer domesticus), 20 saxaul sparrows (Passer ammodendri), and 1 cinnamon sparrow (Passer rutilans), were tested for T. gondii infection, 4 of which were found to be positive for the T. gondii B1 gene by PCR amplification. These positive DNA samples were typed at 10 genetic markers, including 9 nuclear loci, i.e., SAG1, 5′- and 3′-SAG2, alternative SAG2, SAG3, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico. Of these, 3 isolates were genotyped with complete data for all loci, and 2 genotypes (Type I and Type II variant) were identified. This is the first report of genetic typing of T. gondii isolates from wild birds from different regions in China. The results suggest that the Type I and II variant strains are circulating in wild birds in China, and these birds are potential reservoirs for T. gondii transmission.