Changes in Foot Skin Microbiome of Patients with Diabetes Mellitus Using High-Throughput 16S rRNA Gene Sequencing: A Case Control Study from a Single Center
Changes in Foot Skin Microbiome of Patients with Diabetes Mellitus Using High-Throughput 16S rRNA Gene Sequencing: A Case Control Study from a Single Center
复制标题
使用高通量 16S rRNA 基因测序研究糖尿病患者足部皮肤微生物组的变化：单中心病例对照研究
DOI：
10.12659/msm.921440
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发表时间：
2020-05-02
影响因子：
3.1
通讯作者：
Cheng, Biao
中科院分区：
文献类型：
--
作者：
Pang, Mengru;Zhu, Meishu;Cheng, Biao
背景资料：在世界范围内，2型糖尿病相关并发症（包括糖尿病足溃疡（DFU））的治疗给患者和医疗保健系统带来了经济负担。本研究旨在利用高通量165 rRNA基因测序技术研究中国单中心糖尿病患者足部皮肤微生物组的变化。材料/方法：52名参与者分为4组：健康对照组（n=13）;短期糖尿病患者（10年; n=13）。采用高通量16 S核糖体RNA测序技术对取自足弓完整皮肤的拭子进行分析。
Background: Worldwide, the treatment of complications associated with type 2 diabetes mellitus, including diabetic foot ulcer (DFU), results in an economic burden for patients and healthcare systems. This study aimed to use high-throughput 165 rRNA gene sequencing to investigate the changes in foot skin microbiome of patients with diabetes mellitus from a single center in China.Material/Methods: Fifty-two participants were divided into 4 study groups: healthy controls (n=13); patients with short-term di- abetes (10 years; n=13). Swabs were analyzed from the intact skin of the foot arch using high-throughput 16S ribosomal RNA sequencing.Results: Microbiome phylogenic diversity varied significantly between the study groups (whole tree, P