Cycle inhibiting factors (CIFs) are a growing family of functional cyclomodulins present in invertebrate and mammal bacterial pathogens.
Cycle inhibiting factors (CIFs) are a growing family of functional cyclomodulins present in invertebrate and mammal bacterial pathogens.
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DOI：
10.1371/journal.pone.0004855
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Oswald E
中科院分区：
文献类型：
--
作者：
Jubelin G;Chavez CV;Taieb F;Banfield MJ;Samba-Louaka A;Nobe R;Nougayrède JP;Zumbihl R;Givaudan A;Escoubas JM;Oswald E
由肠致病性和肠出血性大肠杆菌产生的周期抑制因子（Cif）是第一个被鉴定为通过III型分泌机制注入宿主细胞的环调节素。Cif引起细胞病变效应，其特征在于G1和G2细胞周期停滞，细胞周期蛋白依赖性激酶抑制剂（CKIs）p21 waf 1/cip 1和p27 kip 1的积累以及肌动蛋白应力纤维的形成。Cif的X射线晶体结构显示它是一个超家族的酶，包括半胱氨酸蛋白酶和乙酰转移酶，共享一个保守的催化三联体的分歧成员。在这里，我们报告的发现和表征的四个CIF同源物编码的不同的病原体或共生细菌分离的脊椎动物或无脊椎动物。来自肠杆菌假结核耶尔森氏菌、发光光杆菌、不对称光杆菌和β-变形杆菌类鼻疽伯克霍尔德氏菌的Cif同系物均诱导与来自致病性大肠杆菌的Cif观察到的相同的细胞病变效应。杆菌虽然这些Cif同源物在一级序列上显著不同，但催化三联体是严格保守的，并且被证明对细胞周期停滞、细胞骨架重组和CKIs积累至关重要。这些结果表明，Cif蛋白在细菌中形成了一个不断增长的环调节蛋白家族，与包括昆虫，线虫和人类在内的非常不同的宿主相互作用。
The cycle inhibiting factor (Cif) produced by enteropathogenic and enterohemorrhagic Escherichia coli was the first cyclomodulin to be identified that is injected into host cells via the type III secretion machinery. Cif provokes cytopathic effects characterized by G1 and G2 cell cycle arrests, accumulation of the cyclin-dependent kinase inhibitors (CKIs) p21waf1/cip1 and p27kip1 and formation of actin stress fibres. The X-ray crystal structure of Cif revealed it to be a divergent member of a superfamily of enzymes including cysteine proteases and acetyltransferases that share a conserved catalytic triad. Here we report the discovery and characterization of four Cif homologs encoded by different pathogenic or symbiotic bacteria isolated from vertebrates or invertebrates. Cif homologs from the enterobacteria Yersinia pseudotuberculosis, Photorhabdus luminescens, Photorhabdus asymbiotica and the β-proteobacterium Burkholderia pseudomallei all induce cytopathic effects identical to those observed with Cif from pathogenic E. coli. Although these Cif homologs are remarkably divergent in primary sequence, the catalytic triad is strictly conserved and was shown to be crucial for cell cycle arrest, cytoskeleton reorganization and CKIs accumulation. These results reveal that Cif proteins form a growing family of cyclomodulins in bacteria that interact with very distinct hosts including insects, nematodes and humans.
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影响因子：
5.6
作者：
Hsu, Yun;Jubelin, Gregory;Taieb, Frederic;Nougayrede, Jean-Philippe;Oswald, Eric;Stebbins, C. Erec
通讯作者：
Stebbins, C. Erec
影响因子：
3.6
作者：
Marchès, O;Ledger, TN;Oswald, E
通讯作者：
Oswald, E
DOI：
10.1073/pnas.182213299
发表时间：
2002-09-17
影响因子：
11.1
作者：
Dale, C;Plague, GR;Moran, NA
通讯作者：
Moran, NA
影响因子：
3.2
作者：
Loukiadis, Estelle;Nobe, Rika;Oswald, Eric
通讯作者：
Oswald, Eric
影响因子：
3.1
作者：
Marchès, O;Nougayrède, JP;Oswald, E
通讯作者：
Oswald, E