Reduction of post injury neointima formation due to 17beta-estradiol and phytoestrogen treatment is not influenced by the pure synthetic estrogen receptor antagonist ICI 182,780 in vitro.
Reduction of post injury neointima formation due to 17beta-estradiol and phytoestrogen treatment is not influenced by the pure synthetic estrogen receptor antagonist ICI 182,780 in vitro.
复制标题
DOI：
10.1186/1471-2261-2-13
复制
发表时间：
2002-08-06
影响因子：
2.1
通讯作者：
Hanke, Hartmut
中科院分区：
文献类型：
--
作者：
Finking, Gerald;Lenz, Christina;Hanke, Hartmut
背景：动物和器官培养实验表明，雌激素对损伤后新生内膜的发育具有有益的抑制作用。这项研究的目的是调查雌激素受体拮抗剂ICI182,780是否影响这种雌激素效应。测定不同浓度的17β-雌二醇和植物雌激素染料木素和大豆苷元的含量。用3F Fogarty导管对胸、腹主动脉进行原位血管损伤。5 mm的片段被随机分组并在培养中保存21天。实验分为三组：1)对照组-20微米ICI-30微米ICI-40微米ICI。2)对照组--20微米ICI--40微米17β-雌二醇-40微米17-β-雌二醇+20微米ICI。3)对照组：20微米ICI-40微米大豆苷元-40微米大豆苷元+20微米ICI-20微米金雀异黄素-20微米金雀异黄素+20微米ICI。结果：1)ICI182,780组未见明显减少新生内膜形成的作用(p=0.05)。2)40微米17β-雌二醇单用(p<；0.0001)和20微米ICI(p<；0.0001)可显著减少新生内膜的形成。3单独应用20微米金雀异黄素(p=0.0083)和联合应用20微米金雀异黄素(p=0.0053)可显著减少新生内膜的形成。结论：雌激素受体拮抗剂ICI182,780不调节雌激素对损伤后新生内膜形成的抑制作用。这些结果不支持这种效应是由血管雌激素受体介导的观点。
BACKGROUND: Animal and organ culture experiments have shown beneficial inhibitory estrogen effects on post injury neointima development. The purpose of this study was to investigate whether such estrogen effects are influenced by the estrogen receptor antagonist ICI 182,780. Different concentrations of 17beta-estradiol and the phytoestrogens genistein and daidzein were tested.METHODS: Female New Zealand White rabbits were benumbed. In situ vascular injury of the thoracic and abdominal aorta was performed by a 3F Fogarty catheter. Segments of 5 mm were randomised and held in culture for 21 days. Three test series were performed: 1) control group--20 microM ICI--30 microM ICI--40 microM ICI. 2) control group--20 microM ICI--40 microM 17beta-estradiol--40 microM 17beta-estradiol + 20 microM ICI. 3) control group--20 microM ICI--40 microM daidzein--40 microM daidzein + 20 microM ICI--20 microM genistein--20 microM genistein + 20 microM ICI. After 21 days the neointima-media-ratio was evaluated.RESULTS: 1) Treatment with ICI 182,780 did not reduce neointima formation significantly (p = 0.05). 2) 40 microM 17beta-estradiol alone (p < 0.0001) and in combination with 20 microM ICI (p < 0.0001) reduced neointima formation significantly. 3) 20 microM genistein alone (p = 0.0083) and combined with 20 microM ICI (p = 0.0053) reduced neointima formation significantly. 40 microM daidzein did not have a significant (p = 0.0637) effect.CONCLUSIONS: The estrogen receptor antagonist ICI 182,780 did not modulate the inhibitory estrogen effects on post injury neointima formation. These results do not support the idea that such effects are mediated by vascular estrogen receptors.