Immune responses induced by recombinant Bacillus subtilis expressing the spike protein of transmissible gastroenteritis virus in pigs
Immune responses induced by recombinant Bacillus subtilis expressing the spike protein of transmissible gastroenteritis virus in pigs
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DOI：
10.1016/j.antiviral.2016.02.003
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发表时间：
2016-07-01
影响因子：
7.6
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Mou, Chunxiao;Zhu, Liqi;Yang, Qian
传染性胃肠炎（Transmissible gastroenteritis，TGE）是一种严重危害哺乳仔猪的疾病，给世界养猪业造成巨大的经济损失。为了研制一种有效、安全、方便的TGE疫苗，我们构建了一株重组枯草芽孢杆菌（B.枯草芽孢杆菌CotGSG）表达猪传染性胃肠炎病毒（TGEV）刺突蛋白（S），探讨其对肠黏膜下树突状细胞（DC）的免疫功能。结果表明，重组B.枯草芽孢杆菌具有募集更多DC到样品B的能力。枯草杆菌CotGSG，迁移到MLN，并诱导免疫应答。用B免疫仔猪。枯草芽孢杆菌CotGSG能显著提高粪便中SIgA的特异性滴度、血清中IgG的滴度和中和抗体。总的来说，我们的结果表明，重组B。表达TGEV S蛋白的枯草杆菌CotGSG能有效诱导DCs的免疫应答，为TGEV疫苗的研究提供了新的思路和方法。(C)© 2016 Elsevier B.V.版权所有。
Transmissible gastroenteritis (TGE) causes severe diarrhea in suckling piglets, results in enormous economic loss in swine-producing areas of the world. To develop an effective, safe, and convenient vaccine for the prevention of TGE, we have constructed a recombinant Bacillus subtilis strain (B. subtilis CotGSG) displaying the transmissible gastroenteritis virus (TGEV) spike (S) protein and discussed its immune function to intestinal submucosal dendritic cells (DCs). Our results showed that the recombinant B. subtilis had the ability to recruit more DCs to sample B. subtilis CotGSG, migrate to MLNs, and induce immune responses. Immunized piglets with B. subtilis CotGSG could significantly elevate the specific SIgA titers in feces, IgG titers and neutralizing antibodies in serum. Collectively, our results suggested that recombinant B. subtilis CotGSG expressing the TGEV S protein could effectively induce immune responses via DCs, and provided a perspective on potential novel strategy and approach that may be applicable to the development of the next generation of TGEV vaccines. (C) 2016 Elsevier B.V. All rights reserved.