Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site.
Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site.
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拉克罗斯病毒 G1 糖蛋白的表位在单个抗原位点内形成重叠的簇。
DOI：
10.1016/0042-6822(85)90283-1
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Gonzalez-Scarano,F
中科院分区：
文献类型：
--
作者：
Najjar,JA;Gentsch,JR;Nathanson,N;Gonzalez-Scarano,F
通过构建中和和非中和单克隆抗体，确定了拉克罗斯布尼亚病毒G1糖蛋白上的抗原位点（F. Gonzalez-Scarano， R. E. Shope， C. H. Calisher和N. Nathanson (1982),Virology120, 42-53）。为了分析单克隆抗体描述的单个表位之间的关系，使用11种中和抗体筛选变异病毒。用中和法和ELISA法检测这些变异病毒对抗g1蛋白单克隆抗体的作用。中和试验将11个表位分为5组，分别为6、2、1、1和1个表位。ELISA试验给出了类似的模式，但也证明了5个表位组中的4个之间的相互关系，表明G1蛋白上可能存在单一的免疫显性抗原位点。当8个非中和性抗g1单克隆抗体在ELISA中检测时，它们属于中和定义的5个表位组中的3个；没有证据表明G1蛋白上存在单独的非中和抗原位点。
Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982),Virology120, 42–53). To analyze the relationship between the individual epitopes delineated by monoclonal antibodies, 11 neutralizing antibodies were used to select variant viruses. These variant viruses were tested against the panel of anti-G1 protein monoclonal antibodies by neutralization and by ELISA. The neutralization tests assigned the 11 epitopes to five groups, consisting of 6, 2, 1, 1, and 1 epitopes. ELISA tests gave a similar pattern, but also demonstrated interrelationships between four of the five epitope groups, suggesting that there may be a single immunodominant antigenic site on the G1 protein. When eight nonneutralizing anti-G1 monoclonal antibodies were tested in ELISA, they fell into three of the five epitope groups defined by neutralization; there was no evidence of a separate nonneutralizing antigenic site on the G1 protein.