CNS gene encoding astrotactin, which supports neuronal migration along glial fibers
CNS gene encoding astrotactin, which supports neuronal migration along glial fibers
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DOI：
10.1126/science.272.5260.417
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发表时间：
1996-04-19
期刊：
影响因子：
56.9
通讯作者：
Hatten, ME
中科院分区：
文献类型：
--
作者：
Zheng, C;Heintz, N;Hatten, ME
脊椎动物中枢神经系统（CNS）的组织发生依赖于胶质细胞引导的有丝分裂后神经元迁移形成神经元板层。先前的研究已经证实神经元蛋白astrotactin在体外小鼠小脑颗粒细胞迁移中起作用。编码astrotactin的基因预测具有三个表皮生长因子重复和两个纤连蛋白III型重复的蛋白质。Astrotactin信使RNA在小脑、海马、大脑和嗅球中的有丝分裂后神经元前体中表达，其中迁移建立层状结构。重组astrotactin肽抗体的Fab片段阻断了体外小脑颗粒神经元沿着星形胶质纤维的迁移。将astrotactin互补DNA转染到3 T3细胞中表明，astrotactin在神经元迁移过程中作为神经元-胶质细胞结合的配体。
Vertebrate central nervous system (CNS) histogenesis depends on glia-guided migration of postmitotic neurons to form neuronal laminae. Previous studies have established that the neuronal protein astrotactin functions in murine cerebellar granule cell migration in vitro. The gene encoding astrotactin predicts a protein with three epidermal growth factor repeats and two fibronectin type III repeats. Astrotactin messenger RNA is expressed in postmitotic neuronal precursors in the cerebellum, hippocampus, cerebrum, and olfactory bulb, where migration establishes laminar structures. Fab fragments of antibodies to a recombinant astrotactin peptide blocked migration of cerebellar granule neurons in vitro along astroglial fibers. Transfection of astrotactin complementary DNA into 3T3 cells indicated that astrotactin acts as a ligand for neuron-glia binding during neuronal migration.