ADSORPTION OF INFLUENZA VIRUS ON CELLS OF THE RESPIRATORY TRACT.
ADSORPTION OF INFLUENZA VIRUS ON CELLS OF THE RESPIRATORY TRACT.
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DOI：
10.1084/jem.78.2.99
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发表时间：
1943-08-01
期刊：
影响因子：
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通讯作者：
Hirst GK
中科院分区：
文献类型：
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作者：
Hirst GK
对流感病毒与雪貂肺细胞的反应进行了研究，结果如下：(1)肺细胞迅速吸附了大量经气管内接种的病毒。(2)在短时间间隔后，肺细胞开始自发地释放吸附的病毒，在乙型流感的情况下，5小时后释放完成75%。(3)与PR8相比，Lee菌株在5 h后从肺细胞中完全释放。(4)细胞释放被吸附的病毒后，表现出不吸附病毒的能力。(5)加热或福尔马林使病毒对小鼠的感染能力消失后，病毒被肺细胞吸附而不释放。除了最后一个特征外，流感病毒与肺细胞之间的相互作用与流感病毒与禽类红细胞之间的相互作用非常相似。在活的雪貂中，吸入的流感病毒也被肺迅速吸附，但在很短的时间内，被吸附的病毒（在灌注和切除肺后）变得非常牢固，无法通过这种方法释放出来。在活的雪貂暴露于接种物24小时后，才能在其体内发现游离病毒。在这些实验和以前的实验的基础上，我们假设，在流感病毒寄生于易感细胞的过程中，一种特定受体物质的破坏——可能涉及酶促反应——可能是必要的初步事件。
A study of the reaction between influenza virus and the cells of the excised and perfused ferret lung has yielded the following results: (1) The cells of the lung rapidly adsorbed large amounts of intratracheally inoculated virus. (2) After a short interval the pulmonary cells began spontaneously to release the adsorbed virus, and in the case of influenza B the release was 75 per cent complete after 5 hours. (3) The Lee strain was more completely released from pulmonary cells after 5 hours than was the PR8 strain. (4) After the cells released the adsorbed virus they appeared incapable of adsorbing virus as before. (5) When the mouse-infecting capacity of the virus had been done away with by heat or formalin, the virus was adsorbed by the pulmonary cells but was not released. In all except the last of the characteristics listed the interaction between influenza virus and the pulmonary cells closely resembles that between influenza virus and avian red blood cells. In the living ferret inhaled influenza virus was also rapidly adsorbed by the lung, but in a very short time the adsorbed virus which at first could be readily eluted (after perfusion and excision of the lung) became so much more firmly fixed as not to be released by this method. Free virus could not be demonstrated in the living ferret until 24 hours after the animal had been exposed to the inoculum. On the basis of these and previous experiments it is postulated that the destruction of a specific receptor substance,—which may involve an enzymatic reaction,—may be a necessary preliminary event in the parasitism of susceptible cells by influenza virus.