The state of water molecules induces changes in the topologies and interactions of G-quadruplex DNA aptamers in hydrated ionic liquid
The state of water molecules induces changes in the topologies and interactions of G-quadruplex DNA aptamers in hydrated ionic liquid
复制标题
水分子状态引起水合离子液体中 G-四链体 DNA 适体拓扑结构和相互作用的变化
DOI：
10.1016/j.molliq.2022.120175
复制
发表时间：
2022
影响因子：
6
通讯作者：
Ikebukuro Kazunori
中科院分区：
文献类型：
--
作者：
Fujita Kyoko;Honda Takuya;Tsukakoshi Kaori;Ohno Hiroyuki;Ikebukuro Kazunori
本文利用水合离子液体研究了水分子的结合态对G-四链体DNA适体的拓扑结构和相互作用的影响。由于细胞内的水分子通常预期存在于结合状态，而不是作为自由分子存在于典型的玻璃介质中，我们提出了水合离子液体作为潜在的候选人，用于控制水分子的状态。已报道水合离子液体溶解蛋白质而不损害其高级结构。本研究采用圆二色性光谱（CD）和酶联寡核苷酸分析法，通过改变水合磷酸二氢胆碱（Hy[ch][dhp]）的含水量，研究了三种G-四链体DNA适体（一种凝血酶结合适体和两种血管内皮生长因子165结合适体）的结构及其与靶分子的相互作用。Hy[ch][dhp]允许G-四链体DNA适体的有效溶解，同时保持它们的结构和对靶分子的结合亲和力。Hy[ch][dhp]的水含量引起CD光谱的变化，表明G-四链体结构的拓扑结构发生了变化。增加的结构稳定性和结合性能表明分子识别和顺利脱水过程中Hy[ch][dhp]通过调节水分子的状态。
Herein, the effects of the bound state of water molecules were investigated on the topologies and interactions of G-quadruplex DNA aptamers by using hydrated ionic liquids (ILs). Since intracellular water molecules are generally expected to exist in the bound state and not as free molecules that exist in the typicalin vitromedia, we proposed hydrated ILs as potential candidates for controlling the state of water molecules. Hydrated ILs have been reported to dissolve proteins without compromising their higher-order structures. In this study, the structures and interactions of three G-quadruplex DNA aptamers (one thrombin-binding aptamer and two vascular endothelial growth factor 165-binding aptamers), with their target molecules were analyzed with circular dichroism (CD) spectroscopy and enzyme-linked oligonucleotide assay by changing the water content of hydrated cholinium dihydrogen phosphate (Hy[ch][dhp]). Hy[ch][dhp] allowed the effective dissolution of G-quadruplex DNA aptamers while maintaining their structure and binding affinity to the target molecule. The water content of Hy[ch][dhp] induced changes in the CD spectra, suggesting changes in the topology of G-quadruplex structure. Increased structural stability and binding properties indicated molecular recognition and smooth dehydration progress in Hy[ch][dhp] via regulation of the state of water molecules.