Patterning cellular motility using an electrochemical technique and a geometrically confined environment
Patterning cellular motility using an electrochemical technique and a geometrically confined environment
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DOI：
10.1021/la0610654
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发表时间：
2006-12-05
期刊：
影响因子：
3.9
通讯作者：
Nishizawa, Matsuhiko
中科院分区：
文献类型：
--
作者：
Kaji, Hirokazu;Kawashima, Takeaki;Nishizawa, Matsuhiko
我们在此描述了一种用于在时间和空间上控制基底上允许的细胞迁移和增殖的模式的方法。使用这种方法，将限制在限定区域内的汇合单层细胞释放到相邻区域中。结合到该方法中的是一种电化学技术，该技术使用扫描微电极在系统表面上绘制区域，然后可以支持细胞迁移和生长。支撑玻璃基板图案化有2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）聚合物的区域，其不受电化学处理的影响并且还稳健地抵抗细胞过度生长，以及当电化学处理时可以从细胞排斥性单独切换到细胞粘附的区域。因此，可以严格定义细胞可以迁移的区域。我们发现，HeLa细胞迁移更迅速的细胞粘附车道的宽度增加，直到宽度约为。达到50 μ m时，迁移率大致保持不变。我们还设计了一个药物测定使用我们的细胞迁移技术。该技术允许细胞仅迁移到限定的区域，因此可能成为评估潜在药物的生物活性的重要工具，因为药物活性和细胞运动性通常直接相关。
We describe herein a method for controlling the pattern of permissible cell migration and proliferation on a substrate in time and space. Using this method, a confluent monolayer of cells that is confined within a defined region is released into a neighboring region. Incorporated into the method is an electrochemical technique that uses a scanning microelectrode to draw regions on the surface of the system that thereafter can support cell migration and growth. The supporting glass substrate is patterned with regions of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer that are not affected by the electrochemical treatment and also robustly resist cellular overgrowth as well as regions that can be individually switched when electrochemically treated from cell repellent to cell adhering. It is therefore possible to strictly define the areas into which cells can migrate. We found that HeLa cells migrate more rapidly as the width of cell-adhering lanes increases until a width of ca. 50 mu m is reached, at which point the migration rate is roughly constant. We also designed a drug assay using our cell migration technique. The technique allows for cell migration only into defined region(s) and therefore may become an important tool for evaluating the biological activity of potential drugs because drug activity and cell motility often directly correlate.