CA-2+ AND NUCLEOTIDE DEPENDENT REGULATION OF VOLTAGE DEPENDENT ANION CHANNELS IN THE PLASMA-MEMBRANE OF GUARD-CELLS
CA-2+ AND NUCLEOTIDE DEPENDENT REGULATION OF VOLTAGE DEPENDENT ANION CHANNELS IN THE PLASMA-MEMBRANE OF GUARD-CELLS
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DOI：
10.1002/j.1460-2075.1990.tb07608.x
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发表时间：
1990-12-01
期刊：
影响因子：
11.4
通讯作者：
RASCHKE, K
中科院分区：
文献类型：
--
作者：
HEDRICH, R;BUSCH, H;RASCHKE, K
利用膜片钳技术，我们发现，在保卫细胞的质膜上的电压依赖性阴离子通道被激活的细胞质中的Ca ~（2+）的存在下，核苷酸的上升。在激活时，这些阴离子通道催化比失活状态高10-20倍的阴离子电流，从而使质膜从K+传导状态转变为阴离子传导状态。通过去极化电压的延长刺激导致阴离子电流的失活（t1/2 = 10- 1/2 s）。我们认为，激活的阴离子通道的Ca 2+和核苷酸是一个关键的事件，在气孔关闭过程中的盐从保卫细胞流出的调节。
Using the patch-clamp technique we discovered that the voltage dependent anion channels in the plasma membrane of guard cells are activated by a rise in cytoplasmic Ca2+ in the presence of nucleotides. Upon activation, these anion channels catalyse anion currents 10-20 times higher than in the inactivated state, thus shifting the plasma membrane from a K+ conducting state to an anion conducting state. Prolonged stimulation by depolarizing voltages results in the inactivation of the anion current (t1/2 = 10-12 s). We suggest that activation of the anion channel by Ca2+ and nucleotides is a key event in the regulation of salt efflux from guard cells during stomatal closure.