Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide with the nonradiometric BACTEC MGIT 960 system
Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide with the nonradiometric BACTEC MGIT 960 system
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DOI：
10.1128/jcm.40.5.1670-1674.2002
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发表时间：
2002-05-01
影响因子：
9.4
通讯作者：
Rüsch-Gerdes, S
中科院分区：
文献类型：
--
作者：
Pfyffer, GE;Palicova, F;Rüsch-Gerdes, S
新型BACTEC MGIT 960吡嗪酰胺（PZA）试剂盒（Becton Dickinson Microbiology Systems，Sparks，Md.）用于检测结核分枝杆菌对PZA的敏感性。将BACTEC MGIT 960系统（Becton Dickinson）产生的结果与BACTEC 460 TB系统获得的结果进行比较。广泛的能力测试（第1阶段）和再现性测试（第11阶段）以及M盲株的敏感性测试。在测试从临床样本分离的58个菌株（IV期）之前，进行来自疾病控制和预防中心的结核病（第111期）。由另外两个实验室作为独立的仲裁者（第V阶段）解决两种BACTEC方法获得的不一致结果后，BACTEC MGIT 960系统与BACTEC 460 TB系统用于I-V阶段菌株PZA检测的总体一致性为96.6%，c.两种系统之间在获得结果的时间上没有统计学显著差异，即，6.8天（BACTEC MGIT 960）对5.4天（BACTEC 460 TB），然而后者不计算生长指数为200的传代培养所需的时间。新的BACTEC MGIT PZA敏感性测试程序同样适用于由液体（MGIT）和固体（Lowenstein-Jensen）培养物制备的接种物。塑料管中的PZA MGIT培养基产生的结果等同于玻璃管中分配的培养基。
The reliability of the novel BACTEC MGIT 960 pyrazinamide (PZA) kit (Becton Dickinson Microbiology Systems, Sparks, Md.) was assessed for testing of susceptibility of Mycobacterium tuberculosis to PZA. Results generated by the BACTEC MGIT 960 system (Becton Dickinson) were compared with those obtained with the BACTEC 460TB system. Extensive proficiency testing (phase 1) and reproducibility testing (phase 11) as well as susceptibility testing of blinded strains of M. tuberculosis from the Centers for Disease Control and Prevention (phase 111) were performed prior to testing 58 strains isolated from clinical specimens (phase IV). After resolution of discrepant results obtained by the two BACTEC methods by two other laboratories which acted as independent arbiters (phase V), overall agreement of the BACTEC MGIT 960 system with the BACTEC 460TB system for PZA testing of phase I-V strains was 96.6%,c. Between the two systems there was no statistically significant difference in time until results were obtained, i.e., 6.8 days (BACTEC MGIT 960) versus 5.4 days (BACTEC 460TB), the latter not counting the time required for a subculture with a growth index of 200, however. The new BACTEC MGIT PZA susceptibility testing procedure works equally well for inocula prepared from liquid (MGIT) and solid (Lowenstein-Jensen) cultures. PZA MGIT medium in plastic tubes yielded results equivalent to medium dispensed in glass tubes.