SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and p21-mediated transcriptional activity.
SAMD4B, a novel SAM-containing protein, inhibits AP-1-, p53- and p21-mediated transcriptional activity.
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DOI：
10.5483/bmbrep.2010.43.5.355
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发表时间：
2010-05
期刊：
影响因子：
3.8
通讯作者：
Na Luo;Guan Li;Yongqing Li;Xiongwei Fan;Yuequn Wang;Xiangli Ye;X. Mo;Junmei Zhou;W. Yuan;M. Tan;Huaping Xie;K. Ocorr;R. Bodmer;Yun Deng;Xiushan Wu
中科院分区：
文献类型：
--
作者：
Na Luo;Guan Li;Yongqing Li;Xiongwei Fan;Yuequn Wang;Xiangli Ye;X. Mo;Junmei Zhou;W. Yuan;M. Tan;Huaping Xie;K. Ocorr;R. Bodmer;Yun Deng;Xiushan Wu
无菌α基序（SAM）是一个公认的蛋白质相互作用结构域，参与多种生物学过程。在这里，我们报告了一个新的基因，SAMD 4 B，它编码一个假定的蛋白质的694个氨基酸与SAM结构域的鉴定和表征。北方杂交和RT-PCR分析表明SAMD 4 B在人胚胎和成人组织中广泛表达。转录活性测定显示SAMD 4 B抑制L 8 G5-荧光素酶的转录活性。SAMD 4 B在哺乳动物细胞中过表达可抑制激活蛋白-1（AP-1）、p53和p21的转录活性，siRNA可解除这种抑制作用。缺失分析表明，SAM结构域是转录抑制的主要区域。结果表明，SAMD 4 B是一个广泛表达的基因，参与AP-1-，p53-和p21-介导的转录信号活性。
The sterile alpha motif (SAM) is a putative protein interaction domain involved in a wide variety of biological processes. Here we report the identification and characterization of a novel gene, SAMD4B, which encodes a putative protein of 694 amino acids with a SAM domain. Northern blot and RT-PCR analysis showed that SAMD4B is widely expressed in human embryonic and adult tissues. Transcriptional activity assays show SAMD4B suppresses transcriptional activity of L8G5-luciferase. Over-expression of SAMD4B in mammalian cells inhibited the transcriptional activities of activator protein-1 (AP-1), p53 and p21, and the inhibitory effects can be relieved by siRNA. Deletion analysis indicates that the SAM domain is the main region for transcriptional suppression. The results suggest that SAMD4B is a widely expressed gene involved in AP-1-, p53-and p21-mediated transcriptional signaling activity.