HEMOGLOBIN DEGRADATION IN THE MALARIA PARASITE PLASMODIUM-FALCIPARUM - AN ORDERED PROCESS IN A UNIQUE ORGANELLE
HEMOGLOBIN DEGRADATION IN THE MALARIA PARASITE PLASMODIUM-FALCIPARUM - AN ORDERED PROCESS IN A UNIQUE ORGANELLE
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DOI：
10.1073/pnas.87.8.2931
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发表时间：
1990-04-01
影响因子：
11.1
通讯作者：
HENDERSON, GB
中科院分区：
文献类型：
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作者：
GOLDBERG, DE;SLATER, AFG;HENDERSON, GB
疟原虫恶性疟原虫将宿主红细胞的血红蛋白作为主要营养来源。我们报道了恶性疟原虫消化泡的纯化以及其中降解过程的特征。通过差速离心和密度梯度分离相结合的方法分离出消化泡。纯的消化泡能够在最适pH为5 - 5.5的条件下将血红蛋白降解为小片段。消化泡中的蛋白水解似乎是一个有序的过程，在其他蛋白水解活性能够有效发挥作用之前，需要一种天冬氨酸蛋白酶将完整的血红蛋白切割开。消化泡中不含有通常在溶酶体中发现的其他水解酶，因此似乎是专门为降解血红蛋白而设计的独特的蛋白水解细胞器。
The malaria parasite Plasmodium falciparum uses host erythrocyte hemoglobin as a major nutrient source. We report the purification of P. falciparum digestive vacuoles and characterization of the degradative process therein. Vacuoles were isolated by a combination of differential centrifugation and density gradient separation. The pure vacuoles were capable of degrading hemoglobin to small fragments with a pH optimum of 5-5.5. Proteolysis in the vacuoles appears to be an ordered process, requiring an aspartic protease to clip intact hemoglobin before other proteolytic activities can function efficiently. The vacuoles do not contain other hydrolases commonly found in lysosomes and therefore appear to be unique proteolytic organelles designed specifically to degrade hemoglobin.