Obesity influences the proteome of periodontal ligament tissues following periodontitis induction in rats.
Obesity influences the proteome of periodontal ligament tissues following periodontitis induction in rats.
复制标题
DOI：
10.1111/jre.12983
复制
发表时间：
2022-03
影响因子：
3.5
通讯作者：
M. E. S. Lopes;C. C. Marcantonio-C.;R. S. de Molon;P. Cerri;C. Salmon;L. S. Mofatto;F. H. Nociti Júnior;J. Deschner;J. Cirelli;A. Nogueira
中科院分区：
文献类型：
--
作者：
M. E. S. Lopes;C. C. Marcantonio-C.;R. S. de Molon;P. Cerri;C. Salmon;L. S. Mofatto;F. H. Nociti Júnior;J. Deschner;J. Cirelli;A. Nogueira
背景和目的为了更好地了解牙周炎进展的分子机制，已经进行了许多研究。人们对肥胖对牙周炎发病和发展的潜在影响越来越感兴趣，但涉及的机制仍有待阐明。本研究旨在确定肥胖对实验性牙周炎大鼠的影响，并确定涉及的新途径。方法将16只Holtzman大鼠分为结扎性牙周炎(P)组和肥胖合并结扎性牙周炎（OP）组，每组8只。高脂肪饮食诱导肥胖70天，而牙周炎诱导20天，在双侧上第一磨牙周围放置棉线。采用显微层析法测定牙槽骨损失，并用半腋窝组织仪测定牙槽骨损失。通过蛋白质组学分析评估牙周膜的蛋白质组成。结果：数据分析（体重、脂肪组织重量和血液检查）证实肥胖诱导，而显微ct和组织学分析证实骨质流失。来自牙周韧带组织（PDL）的蛋白质组分析鉴定出819个蛋白，53个为P组所独有，28个为OP组所独有，738个为共同表达。通过免疫组织化学对选定的蛋白（spondin1、vinculin和TRAP）进行验证。结论组织学上，肥胖对牙周炎所致骨质流失无明显影响。然而，目前的研究结果表明，肥胖会影响实验性牙周炎的PDL蛋白质组，从而可以确定个性化治疗方法的潜在目标。
BACKGROUND AND OBJECTIVES Many studies have been conducted to better understand the molecular mechanism involved with periodontitis progression. There has been growing interest in the potential impact of obesity on periodontitis onset and progression, but the mechanisms involved remain to be elucidated. The present study was designed to determine the impact of obesity on experimentally induced periodontitis in rats and identify novel pathways involved. METHODS Sixteen Holtzman rats were distributed into two groups (n = 8): ligature-induced periodontitis (P) and obesity plus ligature-induced periodontitis (OP). Obesity was induced by a high-fat diet for 70 days, whereas periodontitis was induced for 20 days, with a cotton thread placed around the upper first molars bilaterally. Alveolar bone loss was measured by microtomographic analysis and histologically by histometry on the hemimaxillae. The protein composition of the periodontal ligament was evaluated by proteomic analysis. RESULTS Data analysis (body weight, adipose tissue weight, and blood test) confirmed obesity induction, whereas bone loss was confirmed by micro-CT and histologic analyses. Proteome analysis from the periodontal ligament tissues (PDL) identified 819 proteins, 53 exclusive to the P group, 28 exclusive to the OP group, and 738 commonly expressed. Validation was performed by immunohistochemistry for selected proteins (spondin1, vinculin, and TRAP). CONCLUSION Histologically, it was found that obesity did not significantly affect bone loss resulting from periodontitis. However, the present study's findings indicated that obesity affects the proteome of PDL submitted to experimental periodontitis, allowing for identifying potential targets for personalized approaches.