Co-resistance to different classes of antibiotics among ESBL-producers from aquatic systems
Co-resistance to different classes of antibiotics among ESBL-producers from aquatic systems
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DOI：
10.1016/j.watres.2013.09.021
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发表时间：
2014-01-01
期刊：
影响因子：
12.8
通讯作者：
Henriques, Isabel
中科院分区：
文献类型：
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作者：
Tacao, Marta;Moura, Alexandra;Henriques, Isabel
在这项研究中，我们调查了共同出现的耐药的非β-内酰胺类抗生素头孢噻肟耐药的超广谱β-内酰胺酶（ESBL）生产者（ESBL+）与非ESBL生产者（ESBI-），从水生环境。ESBL+对四环素类、氟喹诺酮类和氨基糖苷类的耐药率较高。18例四环素耐药的ESBL+菌中，泰特（A）检出率为88.9%，泰特（B）检出率为16.7%。耐氟喹诺酮类ESBL+（n = 15）菌株中aacA 4-cr和qnrVC 4分别占26.6%和40%。qnrVC 4基因是首次在假单胞菌和大肠杆菌中检测到。ESBL+和ESBL-的Ⅰ类整合酶基因检出率分别为56.41%和27.67%。鉴定的基因盒阵列赋予对氨基糖苷类（aadA型基因和aacA 4）、甲氧苄啶（dfrA 17）、氯霉素（catB 8）、氟喹诺酮类（qnrVC 4）和β-内酰胺类（B（laox-10））的抗性。用CTX-M生产者进行缀合实验。转接合子对3种或3种以上抗生素表现出多重耐药性，接合质粒被分配到IncF、IncK和Inch 1复制子。结果表明，产ESBL菌株对氨基糖苷类、喹诺酮类和四环素类抗生素的耐药性普遍存在共选择性，这些特征被IncF、IncK和Inch 1接合质粒成功调动。这项研究强调了自然水生系统作为携带多种抗性决定因素的移动的遗传平台的水库的重要性。此外，据我们所知，这构成了IncK：：CTX-M-3在嗜水气单胞菌中的首次观察和IncK质粒在葡萄牙的首次报道。(C)2013爱思唯尔有限公司保留所有权利。
In this study we investigated the co-occurrence of resistance to non-beta-lactams among cefotaxime-resistant extended-spectrum beta-lactamase (ESBL) producers (ESBL+) versus non-ESBL producers (ESBI-), from aquatic environments. Higher prevalence of resistance to tetracycline, fluoroquinolones and aminoglycosides were observed in ESBL+. Among ESBL+ resistant to tetracycline (n = 18), tet(A) was detected in 88.9% and tet(B) in 16.7%. Among fluoroquinolone-resistant-ESBL+ (n = 15), aacA4-cr and qnrVC4 were identified in 26.6% and 40% strains, respectively. The qnrVC4 gene was detected for the first time in Pseudomonas sp. and Escherichia coli. Class 1 integrase genes were detected in 56.41% of ESBL+ and in 27.67% ESBL-. Gene cassette arrays identified conferred resistance to aminoglycosides (aadA-type genes and aacA4), trimethoprim (dfrA17), chloramphenicol (catB8), fluoroquinolones (qnrVC4) and beta-lactams (b(laox-10)). Conjugation experiments were performed with CTX-M-producers. Transconjugants showed multiresistance to 3 or more classes of antibiotics, and conjugative plasmids were assigned to IncF, IncK and Inch1 replicons. Results obtained showed that co-selection of resistance to aminoglycosides, quinolones and tetracyclines is prevalent among ESBL-producers and that these features are successfully mobilized by IncF, IncK and Inch 1 conjugative plasmids. This study reinforces the importance of natural aquatic systems as reservoir of mobile genetic platforms carrying multiple resistance determinants. Moreover, to the best of our knowledge, this constitutes the first observation of IncK::CTX-M-3 in Aeromonas hydrophila and the first report of IncK plasmids in Portugal. (C) 2013 Elsevier Ltd. All rights reserved.