Monitoring acetylcholinesterase level changes under oxidative stress through ESIPT-ICT-based near-infrared fluorescent probe
Monitoring acetylcholinesterase level changes under oxidative stress through ESIPT-ICT-based near-infrared fluorescent probe
复制标题
DOI：
10.1016/j.snb.2023.133392
复制
发表时间：
2023-04
期刊：
影响因子：
--
通讯作者：
Xianzhe Wei;Tong Zhu;Yongsheng Ma;Jianying Sun;G. Zheng;Tingbin Ma;Xiaofeng Yang;Zhi-Yu Song
中科院分区：
文献类型：
--
作者：
Xianzhe Wei;Tong Zhu;Yongsheng Ma;Jianying Sun;G. Zheng;Tingbin Ma;Xiaofeng Yang;Zhi-Yu Song
Oxidative stress plays an important role in pathology, and contributes to a variety of diseases, including inflammation, neurodegenerative diseases, and cancer. Research studies have shown that acetylcholinesterase (AChE) plays a key role in regulating oxidative stress. However, an effective analytical method for real-time monitoring of AChE under oxidative stress is still lacking. Currently, fluorescent probes based on dual sensing mechanisms have attracted great attention by combining and amplifying the advantages of both mechanisms. In this work, two NIR fluorescent probes (SNCN-AEandSNC-AE) that combined ESIPT and ICT processes were designed and synthesized for accurate detection of AChE activity. After the probes reacted with AChE, the strong electron push-pull effect enhanced the ICT process, which further synergized with the ESIPT effect, resulting in a distinct NIR fluorescence signal accompanied by a large Stokes shift. In particular,SNCN-AEshowed better detection performance of fast response, good photostability and low cytotoxicity, which was highly suitable for imaging of endogenous AChE in living system. Importantly,SNCN-AEhad been successfully applied for monitoring AChE under oxidative stress in PC12 cells and zebrafish model. These excellent properties made probes the promising tools for studying oxidative stress and related diseases.
