A new, versatile field immunosensor for environmental pollutants -: Development and proof of principle with TNT, diuron, and atrazine
A new, versatile field immunosensor for environmental pollutants -: Development and proof of principle with TNT, diuron, and atrazine
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DOI：
10.1016/j.bios.2004.10.027
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发表时间：
2005-08-15
影响因子：
12.6
通讯作者：
Kettrup, AA
中科院分区：
文献类型：
--
作者：
Ciumasu, IM;Krämer, PM;Kettrup, AA
本文介绍了一种新型、多功能、便携式、小型化的流动注射免疫传感器，用于现场分析。温控现场样机无需外接电源即可运行6h。生物识别元件是一种被分析物特异性抗体，固定在一个可交换的一次性芯片内的金字塔结构的金表面上，该芯片还包含酶示踪剂和样品储存库。酶示踪剂和分析物之间对抗体抗原结合位点的竞争在最后一步产生化学发光信号，该信号与给定检测范围内分析物的浓度成反比。对硝基芳香剂和杀虫剂的原理证明。2、4、6-三硝基甲苯（TNT）的检测限（DL; IC20）在0.1 μ g l（-1）以下，迪乌隆和阿特拉津的检测限分别在0.2 μ g l（-1）左右。这一发展的重要方面是分析物和酶示踪剂之间的竞争设计，由于非特异性结合和/或发光背景信号而产生的非特异性信号，以及芯片内部的流动模式。(c) 2004 Elsevier B.V.版权所有
This paper presents a new, versatile, portable miniaturized flow-injection immunosensor which is designed for field analysis. The temperature-controlled field prototype can run for 6h without external power supply. The bio-recognition element is an analyte-specific antibody immobilized on a gold surface of pyramidal structures inside an exchangeable single-use chip, which hosts also the enzyme-tracer and the sample reservoirs. The competition between the enzyme-tracer and the analyte for the antigen-binding sites of the antibodies yields in the final step a chemiluminescence signal that is inversely proportional to the concentration of analyte in the given range of detection. A proof of principle is shown for nitroaromatics and pesticides. The detection limits (DL; IC20) reached with the field prototype in the laboratory was below 0.1 mu g l(-1) for 2,4,6-trinitrotoluene (TNT), and about 0.2 mu g l(-1) for diuron and atrazine, respectively. Important aspects in this development were the design of the competition between analyte and enzyme-tracer, the unspecific signal due to unspecific binding and/or luminescence background signal, and the flow pattern inside the chip. (c) 2004 Elsevier B.V. All rights reserved.