Trnp1 organizes diverse nuclear membrane-less compartments in neural stem cells
Trnp1 organizes diverse nuclear membrane-less compartments in neural stem cells
复制标题
Trnp1 在神经干细胞中组织多种无核膜区室
DOI：
10.15252/embj.2019103373
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发表时间：
2020-07-06
期刊：
影响因子：
11.4
通讯作者：
Goetz, Magdalena
中科院分区：
文献类型：
--
作者：
Esgleas, Miriam;Falk, Sven;Goetz, Magdalena
TMF1-regulated nuclear protein 1 (Trnp1) has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding, but its molecular function in the nucleus is still unknown. Here, we show that Trnp1 is a low complexity protein with the capacity to phase separate. Trnp1 interacts with factors located in several nuclear membrane-less organelles, the nucleolus, nuclear speckles, and condensed chromatin. Importantly, Trnp1 co-regulates the architecture and function of these nuclear compartmentsin vitroand in the developing brainin vivo. Deletion of a highly conserved region in the N-terminal intrinsic disordered region abolishes the capacity of Trnp1 to regulate nucleoli and heterochromatin size, proliferation, and M-phase length; decreases the capacity to phase separate; and abrogates most of Trnp1 protein interactions. Thus, we identified Trnp1 as a novel regulator of several nuclear membrane-less compartments, a function important to maintain cells in a self-renewing proliferative state.
